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DHFR Antibody (monoclonal) (M01)
Mouse monoclonal antibody raised against a partial recombinant DHFR.
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IF, E |
|---|---|
| Primary Accession | P00374 |
| Other Accession | BC003584 |
| Reactivity | Human, Rat |
| Host | mouse |
| Clonality | Monoclonal |
| Isotype | IgG2a Kappa |
| Clone Names | 2B10 |
| Calculated MW | 21453 Da |
| Gene ID | 1719 |
|---|---|
| Other Names | Dihydrofolate reductase, DHFR |
| Target/Specificity | DHFR (AAH03584, 88 a.a. ~ 187 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. |
| Dilution | WB~~1:500~1000 |
| Format | Clear, colorless solution in phosphate buffered saline, pH 7.2 . |
| Storage | Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing. |
| Precautions | DHFR Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures. |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
Dihydrofolate reductase converts dihydrofolate into tetrahydrofolate, a methyl group shuttle required for the de novo synthesis of purines, thymidylic acid, and certain amino acids. While the functional dihydrofolate reductase gene has been mapped to chromosome 5, multiple intronless processed pseudogenes or dihydrofolate reductase-like genes have been identified on separate chromosomes. Dihydrofolate reductase deficiency has been linked to megaloblastic anemia.
References
1.Influence of Reduced Folate Carrier and Dihydrofolate Reductase Genes on Methotrexate-Induced Cytotoxicity.Yoon SA, Choi JR, Kim JO, Shin JY, Zhang X, Kang JH.Cancer Res Treat. 2010 Sep;42(3):163-71. Epub 2010 Sep 30.2.Telomere capping in non-dividing yeast cells requires Yku and Rap1.Vodenicharov MD, Laterreur N, Wellinger RJ.EMBO J. 2010 Jul 13. [Epub ahead of print]3.Anticancer drug encapsulated in inorganic lattice can overcome drug resistance.Choi SJ, Choi GE, Oh JM, Oh YJ, Park MC, Choy JH.J. Mater. Chem., 2010, 20, 9463-94694.Deficient BH4 production via de novo and salvage pathways regulates NO responses to cytokines in adult cardiac myocytes.Ionova IA, Vasquez-Vivar J, Whitsett J, Herrnreiter A, Medhora M, Cooley BC, Pieper GM.Am J Physiol Heart Circ Physiol. 2008 Nov;295(5):H2178-87. Epub 2008 Oct 3.
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