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CTSD Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a full length recombinant CTSD.

     
  • IP - CTSD Antibody (monoclonal) (M01) AT1680a
    Immunoprecipitation of CTSD transfected lysate using anti-CTSD monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with CTSD MaxPab rabbit polyclonal antibody.
    detail
  • WB - CTSD Antibody (monoclonal) (M01) AT1680a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (71.06 KDa) .
    detail
  • WB - CTSD Antibody (monoclonal) (M01) AT1680a
    Western Blot analysis of CTSD expression in transfected 293T cell line by CTSD monoclonal antibody (M01), clone 3F12-1B9.

    Lane 1: CTSD transfected lysate (Predicted MW: 44.6 KDa).
    Lane 2: Non-transfected lysate.
    detail
  • IHC - CTSD Antibody (monoclonal) (M01) AT1680a
    Immunoperoxidase of monoclonal antibody to CTSD on formalin-fixed paraffin-embedded human testis. [antibody concentration 1.5 ug/ml]
    detail
  • E - CTSD Antibody (monoclonal) (M01) AT1680a
    Detection limit for recombinant GST tagged CTSD is approximately 3ng/ml as a capture antibody.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC, IP, E
Primary Accession P07339
Other Accession BC016320
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG1 kappa
Clone Names 3F12-1B9
Calculated MW 44552 Da
Additional Information
Gene ID 1509
Other Names Cathepsin D, Cathepsin D light chain, Cathepsin D heavy chain, CTSD, CPSD
Target/Specificity CTSD (AAH16320, 26 a.a. ~ 412 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsCTSD Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)
citation

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Background

This gene encodes a lysosomal aspartyl protease composed of a dimer of disulfide-linked heavy and light chains, both produced from a single protein precursor. This proteinase, which is a member of the peptidase C1 family, has a specificity similar to but narrower than that of pepsin A. Transcription of this gene is initiated from several sites, including one which is a start site for an estrogen-regulated transcript. Mutations in this gene are involved in the pathogenesis of several diseases, including breast cancer and possibly Alzheimer disease.

References

1.Characterization of the Human Gastric Fluid Proteome Reveals Distinct pH-Dependent Protein Profiles: Implications for Biomarker Studies.Kam SY, Hennessy T, Chua SC, Gan CS, Philp R, Hon KK, Lai L, Chan WH, Ong HS, Wong WK, Lim KH, Ling KL, Tan HS, Tan MM, Ho M, Kon OL.J Proteome Res. 2011 Aug 15. [Epub ahead of print]

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$ 350.00
Cat# AT1680a
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Availability: 7-10 days
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