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CSF1 Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a partial recombinant CSF1.

     
  • WB - CSF1 Antibody (monoclonal) (M01) AT1655a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (36.63 KDa) .
    detail
  • WB - CSF1 Antibody (monoclonal) (M01) AT1655a
    Western Blot analysis of CSF1 expression in transfected 293T cell line by CSF1 monoclonal antibody (M01), clone 1A9.

    Lane 1: CSF1 transfected lysate(60.1 KDa).
    Lane 2: Non-transfected lysate.
    detail
  • E - CSF1 Antibody (monoclonal) (M01) AT1655a
    Detection limit for recombinant GST tagged CSF1 is approximately 3ng/ml as a capture antibody.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession P09603
Other Accession BC021117
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG1 Kappa
Clone Names 1A9
Calculated MW 60179 Da
Additional Information
Gene ID 1435
Other Names Macrophage colony-stimulating factor 1, CSF-1, M-CSF, MCSF, Lanimostim, Processed macrophage colony-stimulating factor 1, CSF1
Target/Specificity CSF1 (AAH21117, 33 a.a. ~ 132 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsCSF1 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)
citation

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Background

The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of macrophages. The active form of the protein is found extracellularly as a disulfide-linked homodimer, and is thought to be produced by proteolytic cleavage of membrane-bound precursors. The encoded protein may be involved in development of the placenta. Four transcript variants encoding three different isoforms have been found for this gene.

References

1.miR148b is a major coordinator of breast cancer progression in a relapse-associated microRNA signature by targeting ITGA5, ROCK1, PIK3CA, NRAS, and CSF1.Cimino D, De Pitta C, Orso F, Zampini M, Casara S, Penna E, Quaglino E, Forni M, Damasco C, Pinatel E, Ponzone R, Romualdi C, Brisken C, De Bortoli M, Biglia N, Provero P, Lanfranchi G, Taverna DFASEB J. 2013 Mar;27(3):1223-35. doi: 10.1096/fj.12-214692. Epub 2012 Dec 11.2.Pathway-Based Biomarker Search by High-Throughput Proteomics Profiling of Secretomes.Lawlor K, Nazarian A, Lacomis L, Tempst P, Villanueva J.J Proteome Res. 2009 Mar;8(3):1489-503.

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$ 350.00
Cat# AT1655a
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