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CMAS Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a partial recombinant CMAS.

     
  • IF - CMAS Antibody (monoclonal) (M01) AT1566a
    Immunofluorescence of monoclonal antibody to CMAS on HeLa cell. [antibody concentration 10 ug/ml]
    detail
  • WB - CMAS Antibody (monoclonal) (M01) AT1566a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (36.74 KDa) .
    detail
  • WB - CMAS Antibody (monoclonal) (M01) AT1566a
    CMAS monoclonal antibody (M01), clone 5A2. Western Blot analysis of CMAS expression in PC-12 ( (Cat # AT1566a )
    detail
  • WB - CMAS Antibody (monoclonal) (M01) AT1566a
    CMAS monoclonal antibody (M01), clone 5A2 Western Blot analysis of CMAS expression in Hela S3 NE ( (Cat # AT1566a )
    detail
  • WB - CMAS Antibody (monoclonal) (M01) AT1566a
    CMAS monoclonal antibody (M01), clone 5A2. Western Blot analysis of CMAS expression in Raw 264.7 ( (Cat # AT1566a )
    detail
  • WB - CMAS Antibody (monoclonal) (M01) AT1566a
    CMAS monoclonal antibody (M01), clone 5A2. Western Blot analysis of CMAS expression in NIH/3T3 ( (Cat # AT1566a )
    detail
  • IHC - CMAS Antibody (monoclonal) (M01) AT1566a
    Immunoperoxidase of monoclonal antibody to CMAS on formalin-fixed paraffin-embedded human salivary gland. [antibody concentration 3 ug/ml]
    detail
  • E - CMAS Antibody (monoclonal) (M01) AT1566a
    Detection limit for recombinant GST tagged CMAS is approximately 1ng/ml as a capture antibody.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC, IF, E
Primary Accession Q8NFW8
Other Accession NM_018686
Reactivity Human, Mouse, Rat
Host mouse
Clonality Monoclonal
Isotype IgG2a Kappa
Clone Names 5A2
Calculated MW 48379 Da
Additional Information
Gene ID 55907
Other Names N-acylneuraminate cytidylyltransferase, CMP-N-acetylneuraminic acid synthase, CMP-NeuNAc synthase, CMAS
Target/Specificity CMAS (NP_061156, 164 a.a. ~ 263 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsCMAS Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)
citation

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Background

The enzyme encoded by this gene catalyzes the activation of Neu5Ac to Cytidine 5-prime-monophosphate N-acetylneuraminic acid (CMP-Neu5Ac), which provides the substrate required for the addition of sialic acid. Sialic acids of cell surface glycoproteins and glycolipids play a pivotal role in the structure and function of animal tissues. The pattern of cell surface sialylation is highly regulated during embryonic development, and changes with stages of differentiation. Studies of a similar murine protein suggest that this protein localizes to the nucleus.

References

A C-terminal phosphatase module conserved in vertebrate CMP-sialic acid synthetases provides a tetramerization interface for the physiologically active enzyme. Oschlies M, et al. J Mol Biol, 2009 Oct 16. PMID 19666032.The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Gerhard DS, et al. Genome Res, 2004 Oct. PMID 15489334.Complete sequencing and characterization of 21,243 full-length human cDNAs. Ota T, et al. Nat Genet, 2004 Jan. PMID 14702039.Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. Strausberg RL, et al. Proc Natl Acad Sci U S A, 2002 Dec 24. PMID 12477932.NotI flanking sequences: a tool for gene discovery and verification of the human genome. Kutsenko AS, et al. Nucleic Acids Res, 2002 Jul 15. PMID 12136098.

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$ 350.00
Cat# AT1566a
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