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CDKN1B Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a full length recombinant CDKN1B.

     
  • IF - CDKN1B Antibody (monoclonal) (M01) AT1481a
    Immunofluorescence of monoclonal antibody to CDKN1B on HeLa cell. [antibody concentration 10 ug/ml]
    detail
  • WB - CDKN1B Antibody (monoclonal) (M01) AT1481a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (47.52 KDa) .
    detail
  • WB - CDKN1B Antibody (monoclonal) (M01) AT1481a
    Western Blot analysis of CDKN1B expression in transfected 293T cell line by CDKN1B monoclonal antibody (M01), clone 4B4-E6.

    Lane 1: CDKN1B transfected lysate(22.1 KDa).
    Lane 2: Non-transfected lysate.
    detail
  • IHC - CDKN1B Antibody (monoclonal) (M01) AT1481a
    Immunoperoxidase of monoclonal antibody to CDKN1B on formalin-fixed paraffin-embedded human ovary, clear cell carcinoma tissue. [antibody concentration 5 ug/ml]
    detail
  • E - CDKN1B Antibody (monoclonal) (M01) AT1481a
    Detection limit for recombinant GST tagged CDKN1B is 10 ng/ml as a capture antibody.
    detail
  •  - CDKN1B Antibody (monoclonal) (M01) AT1481a
    Proximity Ligation Analysis of protein-protein interactions between AKT1 and CDKN1B HeLa cells were stained with anti-AKT1 rabbit purified polyclonal 1:1200 and anti-CDKN1B mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC, IF, E
Primary Accession P46527
Other Accession BC001971
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG1 kappa
Clone Names 4B4-E6
Calculated MW 22073 Da
Additional Information
Gene ID 1027
Other Names Cyclin-dependent kinase inhibitor 1B, Cyclin-dependent kinase inhibitor p27, p27Kip1, CDKN1B, KIP1
Target/Specificity CDKN1B (AAH01971, 1 a.a. ~ 198 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsCDKN1B Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)
citation

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Background

This gene encodes a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state.

References

JAB1 expression is associated with inverse expression of p27(kip1) in hepatocellular carcinoma. Qin J, et al. Hepatogastroenterology, 2010 May-Jun. PMID 20698225.CCND1 and CDKN1B polymorphisms and risk of breast cancer. Canbay E, et al. Anticancer Res, 2010 Jul. PMID 20683061.Variation at the NFATC2 Locus Increases the Risk of Thiazolinedinedione-Induced Edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) Study. Bailey SD, et al. Diabetes Care, 2010 Jul 13. PMID 20628086.Analysis of cyclin-dependent kinase inhibitor 1B mutation in Han Chinese women with premature ovarian failure. Wang B, et al. Reprod Biomed Online, 2010 Aug. PMID 20615757.The MENX syndrome and p27: relationships with multiple endocrine neoplasia. Molatore S, et al. Prog Brain Res, 2010. PMID 20541671.

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$ 350.00
Cat# AT1481a
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Availability: 7-10 days
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