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ANXA5 Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a full length recombinant ANXA5.

     
  • IF - ANXA5 Antibody (monoclonal) (M01) AT1151a
    Immunofluorescence of monoclonal antibody to ANXA5 on HeLa cell. [antibody concentration 10 ug/ml]
    detail
  • IP - ANXA5 Antibody (monoclonal) (M01) AT1151a
    Immunoprecipitation of ANXA5 transfected lysate using anti-ANXA5 monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with ANXA5 MaxPab rabbit polyclonal antibody.
    detail
  • WB - ANXA5 Antibody (monoclonal) (M01) AT1151a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (60.94 KDa) .
    detail
  • WB - ANXA5 Antibody (monoclonal) (M01) AT1151a
    ANXA5 monoclonal antibody (M01), clone 1F4-1A5. Western Blot analysis of ANXA5 expression in HeLa.
    detail
  • WB - ANXA5 Antibody (monoclonal) (M01) AT1151a
    Western Blot analysis of ANXA5 expression in transfected 293T cell line by ANXA5 monoclonal antibody (M01), clone 1F4-1A5.

    Lane 1: ANXA5 transfected lysate(35.9 KDa).
    Lane 2: Non-transfected lysate.
    detail
  • IHC - ANXA5 Antibody (monoclonal) (M01) AT1151a
    Immunoperoxidase of monoclonal antibody to ANXA5 on formalin-fixed paraffin-embedded human colon. [antibody concentration 3 ug/ml]
    detail
  • E - ANXA5 Antibody (monoclonal) (M01) AT1151a
    Detection limit for recombinant GST tagged ANXA5 is approximately 0.03ng/ml as a capture antibody.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC, IF, IP
Primary Accession P08758
Other Accession BC001429
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG1 kappa
Clone Names 1F4-1A5
Calculated MW 35937 Da
Additional Information
Gene ID 308
Other Names Annexin A5, Anchorin CII, Annexin V, Annexin-5, Calphobindin I, CBP-I, Endonexin II, Lipocortin V, Placental anticoagulant protein 4, PP4, Placental anticoagulant protein I, PAP-I, Thromboplastin inhibitor, Vascular anticoagulant-alpha, VAC-alpha, ANXA5, ANX5, ENX2, PP4
Target/Specificity ANXA5 (AAH01429, 1 a.a. ~ 320 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsANXA5 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)
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Background

The protein encoded by this gene belongs to the annexin family of calcium-dependent phospholipid binding proteins some of which have been implicated in membrane-related events along exocytotic and endocytotic pathways. Annexin 5 is a phospholipase A2 and protein kinase C inhibitory protein with calcium channel activity and a potential role in cellular signal transduction, inflammation, growth and differentiation. Annexin 5 has also been described as placental anticoagulant protein I, vascular anticoagulant-alpha, endonexin II, lipocortin V, placental protein 4 and anchorin CII. The gene spans 29 kb containing 13 exons, and encodes a single transcript of approximately 1.6 kb and a protein product with a molecular weight of about 35 kDa.

References

1.Transcellular distribution heterogeneity of Annexin A5 represents a protective response to lupus-related thrombophilia: A pilot Proteomics-based study.Zhou D, Luo N, Wu Q, You Y, Zhai Z, Mou Z, Wu Y, Hao F.Biochem Biophys Res Commun. 2012 Apr 6;420(2):357-63. Epub 2012 Mar 8.2.Proteomics and bioinformatics analysis of lovastatin-induced differentiation in ARO cells.Shui HA, Hsia CW, Chen HM, Chang TC, Wang CY.J Proteomics. 2011 Nov 7.3.The Differential Expression of Aqueous Soluble Proteins in Breast Normal and Cancerous Tissues in Relation to Stage and Grade of Patients.Liang S, Singh M, Gam LH.Journal of Biomedicine and Biotechnology doi:10.1155/ 2010/516469

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$ 350.00
Cat# AT1151a
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