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ALDOA Antibody (monoclonal) (M02)

Mouse monoclonal antibody raised against a full length recombinant ALDOA.

     
  • IF - ALDOA Antibody (monoclonal) (M02) AT1116a
    Immunofluorescence of monoclonal antibody to ALDOA on A-431 cell. [antibody concentration 10 ug/ml]
    detail
  • WB - ALDOA Antibody (monoclonal) (M02) AT1116a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (65.78 KDa) .
    detail
  • WB - ALDOA Antibody (monoclonal) (M02) AT1116a
    ALDOA monoclonal antibody (M01), clone 3C12-6D11 Western Blot analysis of ALDOA expression in A-431 ( (Cat # AT1116a )
    detail
  • WB - ALDOA Antibody (monoclonal) (M02) AT1116a
    Western Blot analysis of ALDOA expression in transfected 293T cell line by ALDOA monoclonal antibody (M02), clone 3C12-6D11.

    Lane 1: ALDOA transfected lysate(39 KDa).
    Lane 2: Non-transfected lysate.
    detail
  • SPECIFICATION
  • CITATIONS: 1
  • PROTOCOLS
  • BACKGROUND
  • detail
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IF
Primary Accession P04075
Other Accession BC010660
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG1 kappa
Clone Names 3C12-6D11
Calculated MW 39420 Da
Additional Information
Gene ID 226
Other Names Fructose-bisphosphate aldolase A, Lung cancer antigen NY-LU-1, Muscle-type aldolase, ALDOA, ALDA
Target/Specificity ALDOA (AAH10660.1, 1 a.a. ~ 364 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsALDOA Antibody (monoclonal) (M02) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations ( 0 )

Background

This gene product, Aldolase A (fructose-bisphosphate aldolase) is a glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Three aldolase isozymes (A, B, and C), encoded by three different genes, are differentially expressed during development. Aldolase A is found in the developing embryo and is produced in even greater amounts in adult muscle. Aldolase A expression is repressed in adult liver, kidney and intestine and similar to aldolase C levels in brain and other nervous tissue. Aldolase A deficiency has been associated with myopathy and hemolytic anemia. Alternative splicing of this gene results in multiple transcript variants which encode the same protein.

References

1.Grade-dependent proteomic characterization of kidney cancer.Perroud B, Ishimaru T, Borowsky AD, Weiss RH.Mol Cell Proteomics. 2009 May;8(5):971-85. Epub 2009 Jan 21.

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$ 350.00
Cat# AT1116a
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