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ALDOA Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a full length recombinant ALDOA.

     
  • IF - ALDOA Antibody (monoclonal) (M01) AT1115a
    Immunofluorescence of monoclonal antibody to ALDOA on HeLa cell. [antibody concentration 10 ug/ml]
    detail
  • WB - ALDOA Antibody (monoclonal) (M01) AT1115a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (65.78 KDa) .
    detail
  • WB - ALDOA Antibody (monoclonal) (M01) AT1115a
    ALDOA monoclonal antibody (M01), clone 3D9-6F3. Western Blot analysis of ALDOA expression in human omentum, serous carcinoma.
    detail
  • WB - ALDOA Antibody (monoclonal) (M01) AT1115a
    ALDOA monoclonal antibody (M01), clone 3D9-6F3 Western Blot analysis of ALDOA expression in IMR-32 ( (Cat # AT1115a )
    detail
  • WB - ALDOA Antibody (monoclonal) (M01) AT1115a
    Western Blot analysis of ALDOA expression in transfected 293T cell line by ALDOA monoclonal antibody (M01), clone 3D9-6F3.

    Lane 1: ALDOA transfected lysate (Predicted MW: 39.4 KDa).
    Lane 2: Non-transfected lysate.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IF
Primary Accession P04075
Other Accession BC010660
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG2b Kappa
Clone Names 3D9-6F3
Calculated MW 39420 Da
Additional Information
Gene ID 226
Other Names Fructose-bisphosphate aldolase A, Lung cancer antigen NY-LU-1, Muscle-type aldolase, ALDOA, ALDA
Target/Specificity ALDOA (AAH10660.1, 1 a.a. ~ 364 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsALDOA Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)
citation

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Background

This gene product, Aldolase A (fructose-bisphosphate aldolase) is a glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Three aldolase isozymes (A, B, and C), encoded by three different genes, are differentially expressed during development. Aldolase A is found in the developing embryo and is produced in even greater amounts in adult muscle. Aldolase A expression is repressed in adult liver, kidney and intestine and similar to aldolase C levels in brain and other nervous tissue. Aldolase A deficiency has been associated with myopathy and hemolytic anemia. Alternative splicing of this gene results in multiple transcript variants which encode the same protein.

References

1.Identification of sperm head proteins involved in zona pellucida binding.Petit FM, Serres C, Bourgeon F, Pineau C, Auer J.Hum Reprod. 2013 Jan 25. [Epub ahead of print]2.Fructose-1,6-bisphosphate aldolase A is involved in HaCaT cell migration by inducing lamellipodia formation.Tochio T, Tanaka H, Nakata S, Hosoya H.Journal of Dermatological Science (2008), doi:10.1016/j.jdermsci.2010.02.012

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$ 350.00
Cat# AT1115a
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Availability: 7-10 days
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