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AFP Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a partial recombinant AFP.

     
  • IF - AFP Antibody (monoclonal) (M01) AT1066a
    Immunofluorescence of monoclonal antibody to AFP on HepG2 cell. [antibody concentration 30 ug/ml]
    detail
  • IP - AFP Antibody (monoclonal) (M01) AT1066a
    Immunoprecipitation of AFP transfected lysate using anti-AFP monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with AFP MaxPab rabbit polyclonal antibody.
    detail
  • WB - AFP Antibody (monoclonal) (M01) AT1066a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (37.84 KDa) .
    detail
  • WB - AFP Antibody (monoclonal) (M01) AT1066a
    AFP monoclonal antibody (M01), clone 1G7 Western Blot analysis of AFP expression in HepG2 ( (Cat # AT1066a )
    detail
  • WB - AFP Antibody (monoclonal) (M01) AT1066a
    Western Blot analysis of AFP expression in transfected 293T cell line by AFP monoclonal antibody (M01), clone 1G7.

    Lane 1: AFP transfected lysate(69 KDa).
    Lane 2: Non-transfected lysate.
    detail
  • E - AFP Antibody (monoclonal) (M01) AT1066a
    Detection limit for recombinant GST tagged AFP is approximately 0.03ng/ml as a capture antibody.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IF, IP, E
Primary Accession P02771
Other Accession BC027881
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG
Clone Names 1G7
Calculated MW 68678 Da
Additional Information
Gene ID 174
Other Names Alpha-fetoprotein, Alpha-1-fetoprotein, Alpha-fetoglobulin, AFP, HPAFP
Target/Specificity AFP (AAH27881, 500 a.a. ~ 609 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsAFP Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)
citation

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Background

This gene encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly.

References

1.Immunodevice for simultaneous detection of two relevant tumor markers based on separation of different microparticles by dielectrophoresis.Ramon-Azcon J, Yasukawa T, Mizutani F.Biosens Bioelectron. 2011 Aug 4. [Epub ahead of print]

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$ 350.00
Cat# AT1066a
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