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Anti-Collagen Type I (RABBIT) Antibody Peroxidase Conjugated

Collagen Type I Antibody Peroxidase Conjugated

     
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Product Information
Host Rabbit
Conjugate Peroxidase (Horseradish)
Target Species Mammalian
Reactivity Rat, Human, Mouse, Bovine
Clonality Polyclonal
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC, E, IP, I, LCI
Application Note Anti-COLLAGEN Type I Peroxidase Conjugated Antibody is suitable for western blot, immunoprecipitation, Flow Cytometry, and immunohistochemistry. Researchers should determine optimal titers for applications that are not stated below.
Physical State Lyophilized
Buffer 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
Immunogen Collagen Type I from human and bovine placenta.
Reconstitution Volume 50 µL
Reconstitution Buffer Restore with deionized water (or equivalent)
Stabilizer 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative 0.01% (w/v) Thimerosal
Additional Information
Gene ID 1277
Other Names 1277
Purity This product has been prepared by immunoaffinity chromatography using immobilized antigens.  Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues.  This antibody reacts with most mammalian Type I collagens and has expected cross-reactivity with Type III and negligible cross reactivity with Type II, IV, V or VI collagens. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins has not been tested.
Storage Condition Store vial at 4° C prior to restoration.   Restore with 0.05 mL of deionized water (or equivalent). For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.
Precautions NoteThis product is for research use only and is not intended for therapeutic or diagnostic applications.
Protein Information
Name COL1A1
Function Type I collagen is a member of group I collagen (fibrillar forming collagen).
Cellular Location Secreted, extracellular space, extracellular matrix {ECO:0000255|PROSITE-ProRule:PRU00793}
Tissue Location Forms the fibrils of tendon, ligaments and bones. In bones the fibrils are mineralized with calcium hydroxyapatite
Research Areas
Citations (0)
citation

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Background

COLLAGEN Type I Peroxidase Conjugated Antibody is specific for Collagen Type I. Collagen Type I (Type-I collagen) is the most abundant collagen of the human body. It is present in scar tissue, the end product when tissue heals by repair. It is also found in tendons, the endomysium of myofibrils and the organic part of bone. Anti-collagen Type I antibody is suitable for Cancer research and other general research.

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$ 495.00
Cat# ASR5803
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