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> home > Products > Primary Antibodies > Stem Cells > Anti-Collagen Type IV (RABBIT) Antibody Fluorescein Conjugated
Anti-Collagen Type IV (RABBIT) Antibody Fluorescein Conjugated
Collagen Type IV Antibody Fluorescein Conjugated
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
| Host | Rabbit |
|---|---|
| Conjugate | Fluorescein (FITC) |
| FP Value | 2-7 |
| Target Species | Mammalian |
| Reactivity | Human, Bovine |
| Clonality | Polyclonal |
Application 
| WB, IHC, E, IP, I, LCI |
| Application Note | Anti-Collagen Type IV Fluorescein Conjugated Antibody is suitable for western blotting, IHC and for ELISA. Researchers should determine optimal titers for applications that are not stated below. |
| Physical State | Lyophilized |
| Buffer | 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2 |
| Immunogen | Anti-Collagen Type IV was produced by repeated immunizations with Collagen Type IV from human and bovine placenta. |
| Reconstitution Volume | 50 µL |
| Reconstitution Buffer | Restore with deionized water (or equivalent) |
| Stabilizer | 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free |
| Preservative | 0.01% (w/v) Sodium Azide |
| Gene ID | 1282 |
|---|---|
| Other Names | 1282 |
| Purity | Anti-Collagen Type IV has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities. Typically negligible cross-reactivity against other types of collagens was detected by ELISA against purified standards. Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type IV collagens and has negligible cross-reactivity with Type I, II, III, V and VI collagens. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible. |
| Storage Condition | Store vial at 4° C prior to restoration. Restore with 0.05 mL of deionized water (or equivalent). For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Precautions Note | This product is for research use only and is not intended for therapeutic or diagnostic applications. |
| Name | COL4A1 (HGNC:2202) |
|---|---|
| Function | Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen. |
| Cellular Location | Secreted, extracellular space, extracellular matrix, basement membrane {ECO:0000250|UniProtKB:P02463} |
| Tissue Location | Highly expressed in placenta. |
Research Areas
Citations (0)
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Application Protocols
Provided below are standard protocols that you may find useful for product applications.
Background
Anti-Collagen Type IV Fluorescein Conjugated Antibody detects collagen. Type-IV collagen is a type of collagen found primarily in the basal lamina. It is the major structural element of basal membranes. Anti-Collagen Type IV antibody is ideal for investigators involved in Cell Signaling, Neuroscience and Signal Transduction research.
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$ 495.00
Cat# ASR5778
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