Anti-HUMAN SERUM ALBUMIN (RABBIT) Antibody Fluorescein Conjugated
Human Serum Albumin Antibody Fluorescein Conjugated
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Host | Rabbit |
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Conjugate | Fluorescein (FITC) |
FP Value | 3.7 |
Target Species | Human |
Reactivity | Human |
Clonality | Polyclonal |
Application
| WB, I, LCI |
Application Note | Anti-Human serum Fluorescein has been tested by dot blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. |
Physical State | Lyophilized |
Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
Immunogen | Human Serum Albumin |
Reconstitution Volume | 1.0 mL |
Reconstitution Buffer | Restore with deionized water (or equivalent) |
Stabilizer | 10 mg/ml Polyethylene Glycol (PEG-8000) |
Preservative | 0.01% (w/v) Sodium Azide |
Gene ID | 213 |
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Other Names | 213 |
Purity | This product was prepared from monospecific antiserum by immunoaffinity chromatography using purified human serum albumin. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein and anti-Rabbit Serum. Analysis by SDS-PAGE was used to determine that this preparation is substantially free of aggregates and shows a banding pattern consistent with purified Rabbit IgG. |
Storage Condition | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
Precautions Note | This product is for research use only and is not intended for therapeutic or diagnostic applications. |
Name | ALB |
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Function | Binds water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs (Probable). Its main function is the regulation of the colloidal osmotic pressure of blood (Probable). Major zinc transporter in plasma, typically binds about 80% of all plasma zinc (PubMed:19021548). Major calcium and magnesium transporter in plasma, binds approximately 45% of circulating calcium and magnesium in plasma (By similarity). Potentially has more than two calcium-binding sites and might additionally bind calcium in a non-specific manner (By similarity). The shared binding site between zinc and calcium at residue Asp-273 suggests a crosstalk between zinc and calcium transport in the blood (By similarity). The rank order of affinity is zinc > calcium > magnesium (By similarity). Binds to the bacterial siderophore enterobactin and inhibits enterobactin-mediated iron uptake of E.coli from ferric transferrin, and may thereby limit the utilization of iron and growth of enteric bacteria such as E.coli (PubMed:6234017). Does not prevent iron uptake by the bacterial siderophore aerobactin (PubMed:6234017). |
Cellular Location | Secreted. |
Tissue Location | Plasma. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Anti-Human serum Fluorescein detects Human serum proteins. Serum proteins are those proteins remaining in portion of plasma after coagulation of blood, during which process the plasma protein fibrinogen is converted to fibrin and remains behind in the clot. Anti-Human serum is ideal for investigators involved in Cell Signaling, Cellular Biology and Signal Transduction research.
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