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Anti-ABCB5 (RABBIT) Antibody
ABCB5 Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
| Host | Rabbit |
|---|---|
| Conjugate | Unconjugated |
| Target Species | Human |
| Reactivity | Human |
| Clonality | Polyclonal |
Application 
| WB, IHC, E, I, LCI |
| Application Note | This affinity purified ABCB5 antibody has been tested for use in ELISA, IHC, and western blotting. Specific conditions for reactivity should be optimized by the end user. |
| Physical State | Liquid (sterile filtered) |
| Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Immunogen | ABCB5 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to residues corresponding to an internal region of human ABCB5. |
| Preservative | 0.01% (w/v) Sodium Azide |
| Gene ID | 340273 |
|---|---|
| Other Names | 340273 |
| Purity | This product was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody is specific for human ABCB5 protein. A BLAST analysis was used to suggest partial cross-reactivity with ABCB5 from monkey (85% homology), rat (68% homology) and mouse (62% homology) sources. Cross-reactivity with ABCB5 from other sources has not been determined. |
| Storage Condition | Store Anti-ABCB5 at -20° C prior to opening. Aliquot antibody and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Precautions Note | This product is for research use only and is not intended for therapeutic or diagnostic applications. |
| Name | ABCB5 (HGNC:46) |
|---|---|
| Function | Energy-dependent efflux transporter responsible for decreased drug accumulation in multidrug-resistant cells (PubMed:12960149, PubMed:15205344, PubMed:15899824, PubMed:22306008). Specifically present in limbal stem cells, where it plays a key role in corneal development and repair (By similarity). |
| Cellular Location | Cell membrane; Multi-pass membrane protein {ECO:0000255|PROSITE-ProRule:PRU00441, ECO:0000269|PubMed:12960149} |
| Tissue Location | Expressed by CD133-expressing progenitor cells among epidermal melanocytes (at protein level). Widely expressed with specific expression in pigment cells. Highly expressed in several malignant tissues: highly expressed in clinical melanomas, with low expression in normal skin. In melanoma, marks malignant melanoma- initiating cells (MMIC), in which clinical virulence resides as a consequence of unlimited self-renewal capacity, resulting in inexorable tumor progression and metastasis. Also highly expressed in a number of leukemia cells. Expressed in basal limbal epithelium |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
This antibody is designed, produced, and validated as part of a collaboration between Rockland and the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Anti-ABCB5 Antibody is a ATP-Binding Cassette protein. ABCB5 is a novel member of the human P-glycoprotein family. It functions as a determinant of membrane potential and regulator of cell fusion in physiologic skin progenitor cells. Cell fusion is thought to contribute to tissue differentiation. ABCB5 is predominantly expressed in melanoma cells and is a novel molecular marker for a distinct subset of chemoresistant stem cell phenotype-expressing tumor cells among human epidermal melanocytes. ABCB5 is a rhodamine efflux transporter. This antibody product is intended to be used to confirm cellular localization and expression level of ABCB5. ABCB5 antibody is ideal for Cancer and Signal Transduction research.
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