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Anti-MECT1 (RABBIT) Antibody
MECT1 Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
| Host | Rabbit |
|---|---|
| Conjugate | Unconjugated |
| Target Species | Human |
| Reactivity | Rat, Pufferfish, Human, Mouse, Zebrafish, Bovine |
| Clonality | Polyclonal |
Application 
| WB, E, I, LCI |
| Application Note | This affinity-purified antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 80-95 kDa in size corresponding to MECT1 protein by western blotting in the appropriate cell lysate or extract. This antibody is will react with isoform 1 and 2 of MECT1. |
| Physical State | Liquid (sterile filtered) |
| Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Immunogen | This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 19-34 of human MECT1 protein. |
| Preservative | 0.01% (w/v) Sodium Azide |
| Gene ID | 23373 |
|---|---|
| Other Names | 23373 |
| Purity | This affinity-purified antibody is directed against human MECT1 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest cross reactivity with MECT1 protein from human, mouse, rat, zebrafish, pufferfish and bovine based on 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known. |
| Storage Condition | Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Precautions Note | This product is for research use only and is not intended for therapeutic or diagnostic applications. |
| Name | CRTC1 (HGNC:16062) |
|---|---|
| Function | Transcriptional coactivator for CREB1 which activates transcription through both consensus and variant cAMP response element (CRE) sites. Acts as a coactivator, in the SIK/TORC signaling pathway, being active when dephosphorylated and acts independently of CREB1 'Ser-133' phosphorylation. Enhances the interaction of CREB1 with TAF4. Regulates the expression of specific CREB-activated genes such as the steroidogenic gene, StAR. Potent coactivator of PGC1alpha and inducer of mitochondrial biogenesis in muscle cells. In the hippocampus, involved in late-phase long-term potentiation (L-LTP) maintenance at the Schaffer collateral-CA1 synapses. May be required for dendritic growth of developing cortical neurons (By similarity). In concert with SIK1, regulates the light-induced entrainment of the circadian clock. In response to light stimulus, coactivates the CREB-mediated transcription of PER1 which plays an important role in the photic entrainment of the circadian clock. |
| Cellular Location | Cytoplasm. Nucleus. Note=Cytoplasmic when phosphorylated by SIK or AMPK and when sequestered by 14-3-3 proteins (PubMed:16817901) Translocated to the nucleus on Ser-151 dephosphorylation, instigated by a number of factors including calcium ion and cAMP levels (PubMed:15589160). Light stimulation triggers a nuclear accumulation in the suprachiasmatic nucleus (SCN) of the brain (By similarity) {ECO:0000250|UniProtKB:Q68ED7, ECO:0000269|PubMed:15589160, ECO:0000269|PubMed:16817901} |
| Tissue Location | Highly expressed in adult and fetal brain. Located to specific regions such as the prefrontal cortex and cerebellum. Very low expression in other tissues such as heart, spleen, lung, skeletal muscle, salivary gland, ovary and kidney. |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
This antibody is designed, produced, and validated as part of a collaboration between Rockland and the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. MECT1 (also known as Mucoepidermoid Carcinoma Translocated 1) is a nuclear protein that functions as a transcriptional coactivator for CREB1, which activates transcription through both consensus and variant cAMP response element (CRE) sites. It acts as a coactivator, in the SIK/TORC signaling pathway, being active when dephosphorylated and acts independently of CREB1 Ser-133 phosphorylation. MECT1 enhances the interaction of CREB1 with TAF4. It regulates the expression of specific CREB-activated genes such as the steroidogenic gene, StAR. Mect1 is a potent coactivator of PGC1alpha and inducer of mitochondrial biogenesis in muscle cells. MECT1 is also a coactivator for TAX activation of the human T-cell leukemia virus type 1 (HTLV-1) long terminal repeats (LTR). In the hippocampus, it is involved in late-phase long-term potentiation (L-LTP) maintenance at the Schaffer collateral-CA1 synapses. MECT1 may be required for dendritic growth of developing cortical neurons (By similarity). In concert with SIK1, MECT1 regulates the light-induced entrainment of the circadian clock. In response to light stimulus, it coactivates the CREB-mediated transcription of PER1 which plays an important role in the photic entrainment of the circadian clock. Therefore, it has been found to be important for energy balance and reproduction. Anti-MECT1 (CRTC1) Antibody is useful for researchers interested in glucose energy metabolism, circadian rhythms, and hormone research.
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