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Anti-SLIT-3 (RABBIT) Antibody
SLIT3 Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
| Host | Rabbit |
|---|---|
| Conjugate | Unconjugated |
| Target Species | Human |
| Reactivity | Rat, Human, Mouse, Bovine, Dog |
| Clonality | Polyclonal |
Application 
| WB, E, I, LCI |
| Application Note | This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~ 168 kDa corresponding to full length protein as well as multiple bands corresponding to isoforms of SLIT-3 by western blotting in the appropriate cell lysate or extract. |
| Physical State | Liquid (sterile filtered) |
| Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Immunogen | This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 1155-1180 of Human SLIT-3 protein. |
| Preservative | 0.01% (w/v) Sodium Azide |
| Gene ID | 6586 |
|---|---|
| Other Names | 6586 |
| Purity | This affinity purified antibody is directed against human SLIT-3 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest reactivity with this protein from human, rat, dog, bovine and mouse based on 100% homology for the immunogen sequence. Expect cross reactivity with SLIT-3 from chimpanzee and chicken sources, as only a single amino acid residue change is found within the immunogen sequence (93% positive by BLAST). Cross reactivity with SLIT-3 homologues from other sources has not been determined. |
| Storage Condition | Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Precautions Note | This product is for research use only and is not intended for therapeutic or diagnostic applications. |
| Name | SLIT3 |
|---|---|
| Synonyms | KIAA0814, MEGF5, SLIL2 |
| Function | May act as molecular guidance cue in cellular migration, and function may be mediated by interaction with roundabout homolog receptors. |
| Cellular Location | Secreted. |
| Tissue Location | Predominantly expressed in thyroid. |
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Provided below are standard protocols that you may find useful for product applications.
Background
SLIT-3 (also known as multiple epidermal growth factor-like domain 5 and Slit homolog 3 protein) is a Slit protein. The 'slit' gene has been shown to play a critical role in central nervous system midline formation. In addition to SLIT3 there are two additional human 'slit' homologs, which are termed SLIT1 and SLIT2. Each SLIT gene encodes a putative secreted protein, which contains conserved protein-protein interaction domains including leucine-rich repeats and epidermal growth factor-like motifs, similar to those of the Drosophila protein. SLIT proteins may also participate in the formation and maintenance of the nervous and endocrine systems by protein-protein interactions. Slit-3 is a secreted protein predominantly expressed in thyroid. Multiple isoforms have been reported for this product.
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