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Anti-PC2 [RABBIT] Antibody
PC2 Antibody
![- Anti-PC2 [RABBIT] Antibody ASR5251](/assets/uploads/products/202407/ASR5251-PC2-Antibody-1-IF-4x3.jpg)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
| Host | Rabbit |
|---|---|
| Conjugate | Unconjugated |
| Target Species | Human |
| Reactivity | Human |
| Clonality | Polyclonal |
Application 
| WB, E, IP, I, LCI |
| Application Note | Anti-PC2 antibody has been tested for use in ELISA, WB, and IF. This affinity purified antibody is useful in immunofluorescence staining of cultured cells. In immunofluorescence, this antibody detects the expected discrete nuclear structure that is termed the PcG body, corresponding to the known localization of PC2. This affinity purified antibody is useful in western blotting using transfected cell lysates. Dilutions for western blotting represent a starting point dilution and further optimization may be required. The antibody detects a band of approximately 82 kDa (predicted molecular weight: 61.4 kDa). The antibody has been successfully used to detect FLAG-tagged transfected hPC2 (see figure). It detects a weak band that probably corresponds to endogenous PC2, however, a strong secondary band is also seen at 50kD in all cell lines thus far tested. This suggests that the antibody may also react with another highly expressed ubiquitous protein. Reactivity in other immunoassays is unknown. |
| Physical State | Liquid (sterile filtered) |
| Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Immunogen | This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 90-115 of Human PC2 protein. |
| Preservative | 0.01% (w/v) Sodium Azide |
| Gene ID | 8535 |
|---|---|
| Other Names | 8535 |
| Purity | This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase. Reactivity occurs against human PC2 protein. |
| Storage Condition | Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Precautions Note | This product is for research use only and is not intended for therapeutic or diagnostic applications. |
| Name | CBX4 |
|---|---|
| Function | E3 SUMO-protein ligase which facilitates SUMO1 conjugation by UBE2I (PubMed:12679040). Involved in the sumoylation of HNRNPK, a p53/TP53 transcriptional coactivator, hence indirectly regulates p53/TP53 transcriptional activation resulting in p21/CDKN1A expression. Monosumoylates ZNF131 (PubMed:22825850). |
| Cellular Location | Nucleus. Nucleus speckle. Note=Localization to nuclear polycomb bodies is required for ZNF131 sumoylation (PubMed:22467880). Localized in distinct foci on chromatin (PubMed:18927235) |
| Tissue Location | Ubiquitous. |
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Provided below are standard protocols that you may find useful for product applications.
Background
PC2 is the human homolog of the Drosophila 'Polycomb' (Pc) protein which has been identified as a gene family member associated with a cellular memory system that is responsible for the inheritance of gene activity by progeny cells. The human Pc homolog gene is more closely related to a Xenopus Pc homolog, XPc, than to a previously described human Pc homolog, CBX2 (hPc1). However, the hPc2 and CBX2/hPc1 proteins are shown to colocalize in interphase nuclei of human U-2 OS osteosarcoma cells, suggesting that the proteins are part of a common protein complex. The human protein is believed to function as a repressor of proto-oncogene activity and that interference with hPc2 function can lead to depression of proto-oncogene transcription and subsequently to cellular transformation. Other reports describe PC2 as a protein that has SUMO E3 activity for the corepressors CtBP and CtBP2.
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