Anti-AP3D1 (GOAT) Antibody
AP3D1 Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Host | Goat |
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Conjugate | Unconjugated |
Target Species | Human |
Reactivity | Human |
Clonality | Polyclonal |
Application
| WB, E, IP, I, LCI |
Application Note | This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 130 kDa in size corresponding to AP3D1 by western blotting in the appropriate cell lysate or extract. Multiple isoforms exist for this protein. |
Physical State | Liquid (sterile filtered) |
Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
Immunogen | This affinity purified antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 675-700 of Human AP3D1. |
Preservative | 0.01% (w/v) Sodium Azide |
Gene ID | 8943 |
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Other Names | 8943 |
Purity | This affinity purified antibody is directed against human AP3D1. The product was affinity purified from antiserum by immunoaffinity purification. A BLAST analysis was used to suggest reactivity with this protein from human, chimpanzee and chicken based on 100% homology for the immunogen sequence. Cross reactivity with AP3D1 protein from mouse, rat, dog and cow may occur as sequence homology varies by only one amino acid residue in this sequence as indicated by BLAST analysis. Reactivity with AP3D1 proteins from other sources is not known. |
Storage Condition | Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
Precautions Note | This product is for research use only and is not intended for therapeutic or diagnostic applications. |
Name | AP3D1 |
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Function | Part of the AP-3 complex, an adaptor-related complex which is not clathrin-associated. The complex is associated with the Golgi region as well as more peripheral structures. It facilitates the budding of vesicles from the Golgi membrane and may be directly involved in trafficking to lysosomes. Involved in process of CD8+ T- cell and NK cell degranulation (PubMed:26744459). In concert with the BLOC-1 complex, AP-3 is required to target cargos into vesicles assembled at cell bodies for delivery into neurites and nerve terminals (By similarity). |
Cellular Location | Cytoplasm. Golgi apparatus membrane; Peripheral membrane protein; Cytoplasmic side |
Tissue Location | Present in all adult tissues examined with the highest levels in skeletal muscle, heart, pancreas and testis |
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Provided below are standard protocols that you may find useful for product applications.
Background
AP3D1 (also known as Delta-adaptin 3, AP-3 complex delta subunit and Delta-adaptin) is a subunit of the AP3 adaptor-like complex, which is not associated with clathrin. The AP3D1 subunit is implicated in intracellular biogenesis and trafficking of pigment granules and possibly platelet dense granules and neurotransmitter vesicles.
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