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Anti-Urm1 (RABBIT) Antibody
Urm1 Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
| Host | Rabbit |
|---|---|
| Conjugate | Unconjugated |
| Target Species | Yeast |
| Reactivity | Yeast |
| Clonality | Polyclonal |
Application 
| WB, E, I, LCI |
| Application Note | This purified polyclonal antibody reacts with yeast Urm1 by western blot and ELISA. Although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation. This antibody using the specified conditions may recognize other prominent intrinsic bands (UBLs or their conjugates). Other intrinsic bands are readily detectable in yeast lysates at lower antibody dilutions. For immunoblotting a 12 kDa band corresponding to yeast Urm1 is detected. Most yeast cell lysates can be used as a positive control without induction or stimulation. |
| Physical State | Lyophilized |
| Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Immunogen | This purified antibody was prepared from rabbit serum after repeated immunizations with recombinant yeast Urm1 protein. |
| Reconstitution Volume | 100 µL |
| Reconstitution Buffer | Restore with deionized water (or equivalent) |
| Preservative | 0.01% (w/v) Sodium Azide |
| Gene ID | 854809 |
|---|---|
| Other Names | 854809 |
| Purity | This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum. |
| Storage Condition | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Precautions Note | This product is for research use only and is not intended for therapeutic or diagnostic applications. |
| Name | URM1 {ECO:0000255|HAMAP-Rule:MF_03048} |
|---|---|
| Function | Acts as a sulfur carrier required for 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). Serves as sulfur donor in tRNA 2-thiolation reaction by being thiocarboxylated (-COSH) at its C-terminus by the MOCS3 homolog UBA4. The sulfur is then transferred to tRNA to form 2-thiolation of mcm(5)S(2)U. Prior mcm(5) tRNA modification by the elongator complex is required for 2-thiolation (By similarity) (PubMed:18664566, PubMed:18755837, PubMed:19017811, PubMed:19145231, PubMed:19151091). Also acts as a ubiquitin-like protein (UBL) that is covalently conjugated via an isopeptide bond to lysine residues of target proteins such as AHP1 (PubMed:10713047, PubMed:14551258, PubMed:14555475, PubMed:21209336, PubMed:32004955). Conjugation does not depend on the canonical cascade of E2 ubiquitin-conjugating enzymes and/or E3 ligases. The conjugation reaction requires a thiocarboxylated C- terminus of URM1 and a peroxidatic cysteine in the target protein, as the sulfur atom of the URM1 thiocarboxyl group is transferred to redox- active cysteine residues in the target protein (PubMed:36102610). Oxidative stress specifically induces the formation of UBL-protein conjugates (PubMed:21209336). Covalent modification with URM1 promotes the phase separation of a wide range of proteins into condensates like stress granules (PubMed:38942013). Indirectly involved in regulation of budding and haploid invasive growth (PubMed:14551258). |
| Cellular Location | Cytoplasm. Nucleus. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Ubiquitin-like proteins fall into two classes: the first class, ubiquitin-like modifiers (UBLs) function as modifiers in a manner analogous to that of ubiquitin. Examples of UBLs are SUMO, Rub1 (also called Nedd8), Apg8 and Apg12. Proteins of the second class include parkin, RAD23 and DSK2, are designated ubiquitin-domain proteins (UDPs). These proteins contain domains that are related to ubiquitin but are otherwise unrelated to each other. In contrast to UBLs, UDPs are not conjugated to other proteins. Urm1 is a newly identified ubiquitin related modifier. Urm 1 is a 99-amino acid protein terminated with glycine-glycine. Target proteins are conjugated to Urm1 via its C-terminal glycine. Initially Urm1 forms a thioester with a novel E1-like protein, Uba4.
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