Anti-PLASMINOGEN (Human Plasma) (GOAT) Antibody Biotin Conjugated
Plasminogen Antibody Biotin Conjugated
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Host | Goat |
---|---|
Conjugate | Biotin |
Target Species | Human |
Reactivity | Human |
Clonality | Polyclonal |
Application
| WB, E, I, LCI |
Application Note | Anti-Plasminogen antibody has been tested by ELISA and western blot. This antibody is assayed against 1.0 ug of Plasminogen in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product. |
Physical State | Lyophilized |
Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
Immunogen | Plasminogen [Human Plasma] |
Reconstitution Volume | 100 µL |
Reconstitution Buffer | Restore with deionized water (or equivalent) |
Stabilizer | 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free |
Preservative | 0.01% (w/v) Sodium Azide |
Gene ID | 5340 |
---|---|
Other Names | 5340 |
Purity | Anti-Plasminogen antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Plasminogen [Human Plasma]. Cross reactivity against Plasminogen from other sources is unknown. |
Storage Condition | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
Precautions Note | This product is for research use only and is not intended for therapeutic or diagnostic applications. |
Name | PLG |
---|---|
Function | Plasmin dissolves the fibrin of blood clots and acts as a proteolytic factor in a variety of other processes including embryonic development, tissue remodeling, tumor invasion, and inflammation. In ovulation, weakens the walls of the Graafian follicle. It activates the urokinase-type plasminogen activator, collagenases and several complement zymogens, such as C1 and C5. Cleavage of fibronectin and laminin leads to cell detachment and apoptosis. Also cleaves fibrin, thrombospondin and von Willebrand factor. Its role in tissue remodeling and tumor invasion may be modulated by CSPG4. Binds to cells. |
Cellular Location | Secreted. Note=Locates to the cell surface where it is proteolytically cleaved to produce the active plasmin. Interaction with HRG tethers it to the cell surface |
Tissue Location | Present in plasma and many other extracellular fluids. It is synthesized in the liver |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
info@abcepta.com, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
Background
Anti-Plasminogen antibody recognizes plasminogen, a precursor of plasmin. Plasmin is a serine protease that degrades fibrin clots and involved in embryonic development, tissue remodeling, tumor invasion, and inflammation. In addition to fibrin, Plasmin can also cleave fibronectin, thrombospondin, laminin, and von Willebrand factor. Plasmin can activate other zymogens such as tissue plasminogen activator, urokinase plasminogen activator, kallikrein, and factor XII. Deficiency in plasminogen increases susceptibility to thrombosis.
If you have used an Abcepta product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at tech@abcepta.com.