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Anti-BOVINE ALBUMIN (RABBIT) Antibody (BSA)

Bovine Serum Albumin Antibody BSA

     
  •  - Anti-BOVINE ALBUMIN (RABBIT) Antibody (BSA) ASR3838
    Western Blot of Rabbit anti-Bovine Serum Albumin antibody. Lane 1: BSA (p/n 001-0133). Load: 50 ng per lane. Primary antibody: BSA antibody at 1:1,000 for overnight at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody (p/n 611-103-122) at 1:40,000 for 30 min at RT. Block: 5% BLOTTO-TBS for 30 min at RT. Predicted/Observed size: 66 kDa, 66 kDa for BSA. Other band(s): None.
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Product Information
Host Rabbit
Conjugate Unconjugated
Target Species Bovine
Reactivity Bovine
Clonality Polyclonal
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC, E, I, LCI
Application Note Anti-Bovine Albumin antibody has been tested in ELISA and western blot and is suitable for IHC. The antibody may be suitable for other applications, although no supporting data is available.
Physical State Lyophilized
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Immunogen Anti-Bovine Serum Albumin antibody was produced by repeated immunizations with Bovine Serum Albumin.
Reconstitution Volume 2.0 mL
Reconstitution Buffer Restore with deionized water (or equivalent)
Preservative 0.01% (w/v) Sodium Azide
Additional Information
Gene ID 280717
Other Names 280717
Purity Bovine Serum Albumin antibody is supplied in the form of monospecific antiserum prepared by a delipidation and defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, purified and partially purified Albumin [Bovine Serum].  Cross reactivity against Albumin from other tissues and species may occur but have not been specifically determined.
Storage Condition Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Precautions NoteThis product is for research use only and is not intended for therapeutic or diagnostic applications.
Protein Information
Name ALB
Function Binds water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc (By similarity). Major calcium and magnesium transporter in plasma, binds approximately 45% of circulating calcium and magnesium in plasma (Probable). Potentially has more than two calcium-binding sites and might additionally bind calcium in a non-specific manner (PubMed:22677715). The shared binding site between zinc and calcium at residue Asp-272 suggests a crosstalk between zinc and calcium transport in the blood (Probable). The rank order of affinity is zinc > calcium > magnesium (Probable). Binds to the bacterial siderophore enterobactin and inhibits enterobactin- mediated iron uptake of E.coli, and may thereby limit the utilization of iron and growth of enteric bacteria such as E.coli (PubMed:6234017). Does not prevent iron uptake by the bacterial siderophore aerobactin (PubMed:6234017).
Cellular Location Secreted.
Tissue Location Plasma.
Research Areas
Citations (0)
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Background

Anti-Bovine Serum Albumin antibody detects BSA. Serum albumin is the main protein of plasma. It has a good binding capacity for water, Ca2+, Na+, K+, fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. ALB is the major zinc transporter in plasma; it typically binds about 80% of all plasma zinc. BSA antibody is ideal for investigators involved in Cell Signaling, Neuroscience and Signal Transduction research.

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$ 285.00
Cat# ASR3838
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