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Anti-APC2 (C-terminal specific) (RABBIT) Antibody
APC2 Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
| Host | Rabbit |
|---|---|
| Conjugate | Unconjugated |
| Target Species | Human |
| Reactivity | Human |
| Clonality | Polyclonal |
Application 
| WB, IHC, E, I, LCI |
| Application Note | This antibody reacts with human APC2 by western blot and immunoprecipitation. The antibody immunoprecipitates in vitro translated protein and protein from overexpressing cell lysates (using HeLa and NIH-3T3, and others). Coimmunoprecipitation of related proteins (APC11) does occur. A 93.8 kDa band corresponding to human APC2 is detected. Most cell lines or tissues expressing APC2 can be used as a positive control. Researchers should determine optimal titers for other applications. |
| Physical State | Liquid (sterile filtered) |
| Immunogen | This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 810-822 of Human APC2 (C-terminal) coupled to KLH. |
| Preservative | 0.01% (w/v) Sodium Azide |
| Gene ID | 29882 |
|---|---|
| Other Names | 29882 |
| Purity | This product is monospecific antiserum processed by delipidation and defibrination followed by sterile filtration. This product reacts with human APC2. Cross reactivity may also occur with APC2 from other sources. Sufficient sequence differences exist to suggest that this antibody would not react with other RING box proteins such as ROC1 and ROC2. |
| Storage Condition | Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Precautions Note | This product is for research use only and is not intended for therapeutic or diagnostic applications. |
| Name | ANAPC2 |
|---|---|
| Synonyms | APC2, KIAA1406 |
| Function | Together with the RING-H2 protein ANAPC11, constitutes the catalytic component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle (PubMed:11739784, PubMed:18485873). The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains (PubMed:11739784, PubMed:18485873). The APC/C complex catalyzes assembly of branched 'Lys-11'-/'Lys-48'-linked branched ubiquitin chains on target proteins (PubMed:29033132). The CDC20-APC/C complex positively regulates the formation of synaptic vesicle clustering at active zone to the presynaptic membrane in postmitotic neurons (By similarity). CDC20-APC/C-induced degradation of NEUROD2 drives presynaptic differentiation (By similarity). |
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Provided below are standard protocols that you may find useful for product applications.
Background
APC2, also known as Anaphase promoting complex subunit 2, APC2, Cyclosome subunit 2, and ANAPC2, is a component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/cyclosome protein complex promotes metaphase-anaphase transition by ubiquitinating its specific substrates such as mitotic cyclins and anaphase inhibitors, which are subsequently degraded by the 26S proteasome. Biochemical studies have shown that the vertebrate APC contains at least eleven subunits. The composition of APC is highly conserved in organisms from yeast to humans. APC2 is a cullin family member that interacts through the cullin domain with ANAPC11 and UBCH10.
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