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Anti-Mouse IgG (gamma 1, 2a, 2b and 3 chain) (ATTO 550 Conjugated) Secondary Antibody
Rabbit Polyclonal, ATTO 550
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| Description | Anti-MOUSE IgG (gamma 1, 2a, 2b and 3 chain) (RABBIT) Antibody ATTO 550 Conjugated |
|---|---|
| Host | Rabbit |
| Conjugate | ATTO 550 |
| FP Value | 1.5 moles ATTO 550 per mole of IgG |
| Target Species | Mouse |
| Clonality | Polyclonal |
Application 
| WB, IF |
| Application Note | FLISA >1:20,000 IF Microscopy >1:5,000 Western Blot >1:10,000 |
| Physical State | Lyophilized |
| Host Isotype | IgG |
| Target Isotype | IgG (gamma 1, 2a, 2b and 3 chain) |
| Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Immunogen | highly purified mouse IgG gamma 1, gamma 2a, gamma 2b and gamma 3 proteins |
| Reconstitution Volume | 500 µL |
| Reconstitution Buffer | Restore with deionized water (or equivalent) |
| Stabilizer | 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free |
| Preservative | 0.01% (w/v) Sodium Azide |
| Shipping Condition | Ambient |
|---|---|
| Purity | Mouse IgG (gamma 1, 2a, 2b and 3 chain) Antibody ATTO 550 was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. This product shows balanced reactivity to Mouse IgG1, IgG2a, IgG2b and IgG3 proteins and is suitable to screen IgG class hybridoma clones. Minimal cross reactivity is observed against other Mouse immunoglobulin classes or light chain proteins. |
| Storage Condition | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Precautions Note | This product is for research use only and is not intended for therapeutic or diagnostic applications. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Anti-Mouse IgG (gamma 1, 2a, 2b and 3 chain) conjugated to ATTO 550 is designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
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