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Anti-MOUSE IgG2a (ATTO 647N Conjugated) Pre-adsorbed Secondary Antibody
Goat Polyclonal, ATTO 647N
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- BACKGROUND
| Description | Anti-MOUSE IgG2a (Gamma 2a chain) (GOAT) Antibody ATTO 647N Conjugated (Min Cross Bv, Hu, and Rb Serum Proteins) |
|---|---|
| Host | Goat |
| Conjugate | ATTO 647N |
| FP Value | 1.3 moles ATTO 647N per mole of IgG |
| Target Species | Mouse |
| Reactivity | Mouse |
| Clonality | Polyclonal |
Application 
| WB, IF, FC |
| Application Note | FLISA >1:20,000 IF Microscopy >1:5,000 FlowCytometry 1:500-1:2,500 Western Blot 1:4,000-1:20,000 |
| Physical State | Lyophilized |
| Host Isotype | IgG |
| Target Isotype | IgG2a |
| Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Immunogen | Mouse IgG2a heavy chain |
| Reconstitution Volume | 500 µL |
| Reconstitution Buffer | Restore with deionized water (or equivalent) |
| Stabilizer | 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free |
| Preservative | 0.01% (w/v) Sodium Azide |
| Shipping Condition | Ambient |
|---|---|
| Purity | MOUSE IgG2a Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse Serum and Mouse IgG2a. Specificity was confirmed by ELISA at less than 1% cross-reactivity against other mouse heavy or light chain isotypes. No reaction was observed against Bovine, Human, and Rabbit Serum Proteins. Specificity was confirmed by ELISA at less than 1% of target signal. |
| Storage Condition | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Precautions Note | This product is for research use only and is not intended for therapeutic or diagnostic applications. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Mouse IgG2a secondary antibody is available in a variety of formats. ATTO dye conjugated antibodies are designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
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