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Anti-Sheep IgG F(c) (Alkaline Phosphatase Conjugated) Pre-Adsorbed Secondary Antibody

Rabbit Polyclonal, Alkaline Phosphatase (Calf Intestine)

     
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Product Information
Description Anti-SHEEP IgG F(c) (RABBIT) Antibody Alkaline Phosphatase Conjugated (Min X Human Serum Proteins)
Host Rabbit
Conjugate Alkaline Phosphatase (Calf Intestine)
Target Species Sheep
Clonality Polyclonal
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC, E
Application Note ELISA 1:2,000-1:10,000
Western Blot 1:500-1:2,500
Immunohistochemistry 1:200-1:1,000
Physical State Liquid (sterile filtered)
Host Isotype IgG
Target Isotype IgG F(c)
Buffer 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0
Immunogen Sheep IgG F(c) fragment
Stabilizer 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative 0.01% (w/v) Sodium Azide
Additional Information
Shipping Condition Wet Ice
Purity This product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Sheep IgG, Sheep IgG F(c) and Sheep Serum.  No reaction was observed against Sheep IgG F(ab’)2or Human Serum Proteins..
Storage Condition Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Precautions NoteThis product is for research use only and is not intended for therapeutic or diagnostic applications.
Research Areas
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$ 295.00
Cat# ASR3138
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