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Anti-Goat IgG (Phycoerythrin Conjugated) Secondary Antibody

Donkey Polyclonal, R-Phycoerythrin (RPE)

     
  • WB - Anti-Goat IgG  (Phycoerythrin Conjugated) Secondary Antibody ASR3085
    Western blot of Phycoerythrin conjugated Donkey Anti-Goat IgG Secondary antibody. Lane 1: Goat IgG. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Phycoerythrin donkey secondary antibody at 1:1,000 for 60 min at RT. Blocking: MB-070 for 30 min at RT. Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Goat IgG. Other band(s): None.
    detail
  • DB - Anti-Goat IgG  (Phycoerythrin Conjugated) Secondary Antibody ASR3085
    Dot Blot of Donkey anti-Goat IgG Antibody Phycoerythrin Conjugated. Antigen: Goat IgG. Load: Lane 1 - 100 ng Lane 2 - 33.3 ng Lane 3 - 11.1 ng Lane 4 - 3.70 ng Lane 5 - 1.23 ng. Primary antibody: none. Secondary antibody: Donkey anti-Goat IgG Antibody Phycoerythrin Conjugated at 1:1,000 for 60 min at RT. Block: MB-070 for 1 HR at RT.
    detail
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Product Information
Description Anti-GOAT IgG (DONKEY) Antibody Phycoerythrin conjugate
Host Donkey
Conjugate R-Phycoerythrin (RPE)
Target Species Goat
Clonality Polyclonal
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
IF, FC
Application Note IF Microscopy 1:100-1:250
FlowCytometry 1:100-1:250
FLOW1:100-1:250
Physical State Lyophilized
Host Isotype IgG
Target Isotype IgG (H&L)
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Immunogen Goat IgG whole molecule
Species of Origin donkey
Reconstitution Volume 1.0 mL
Stabilizer 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative 0.01% (w/v) Sodium Azide
Additional Information
Shipping Condition Wet Ice
Purity Anti-Goat IgG was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Donkey Serum, Goat IgG and Goat Serum.  No reaction was observed against Chicken, Guinea Pig, Hamster, Horse, Mouse, Human, Rabbit and Rat Serum Proteins.
Storage Condition Store vial at 4° C prior to opening. Dilute only prior to immediate use. Do not freeze after reconstitution. Store reagent in the dark. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis.
Precautions NoteThis product is for research use only and is not intended for therapeutic or diagnostic applications.
Research Areas
Citations (0)
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Background

Goat IgG antibody recognizes IgG heavy and light chains. Anti-Goat IgG antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Donkey Serum, Goat IgG and Goat Serum. No reaction was observed against Chicken, Guinea Pig, Hamster, Horse, Mouse, Human, Rabbit and Rat Serum Proteins. Anti-Goat IgG (H&L) antibody is ideal for investigators involved in serum component protein research.

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$ 292.00
Cat# ASR3085
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