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F(ab')2 Anti-Rabbit IgG (H&L) (Phycoerythrin Conjugated) Secondary Antibody

Goat Polyclonal, R-Phycoerythrin (RPE)

     
  •  - F(ab')2  Anti-Rabbit IgG (H&L)  (Phycoerythrin Conjugated) Secondary Antibody ASR2871
    Dot Blot of F(ab)2 Goat anti-Rabbit IgG Antibody Phycoerythrin Conjugated. Antigen: Row 1: Rabbit IgG Row 2: Goat IgG Row 3: Mouse IgG Load: Lane 1 - 200ng Lane 2 - 66.7ng Lane 3 - 22.2ng Lane 4 - 7.4ng Lane 5 - 2.5ng Primary antibody: N/A Secondary antibody: F(ab)2 Goat anti-Rabbit IgG Antibody Phycoerythrin Conjugated at 1:1,000 for 60 min at RT. Block: MB-070 for 1 hour at RT
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Product Information
Description F(ab')2 Anti-RABBIT IgG [H&L] (GOAT) Antibody Phycoerythrin conjugated
Host Goat
Conjugate R-Phycoerythrin (RPE)
Target Species Rabbit
Clonality Polyclonal
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
IF, FC
Application Note IF Microscopy 1:100-1:250
FlowCytometry 1:100-1:250
FLOW1:100-1:250
Physical State Lyophilized
Host Isotype IgG F(ab')2
Target Isotype IgG (H&L)
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Immunogen Rabbit IgG whole molecule
Reconstitution Volume 1.0 mL
Reconstitution Buffer Restore with deionized water (or equivalent)
Stabilizer 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative 0.01% (w/v) Sodium Azide
Additional Information
Shipping Condition Ambient
Purity This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit  IgG coupled to agarose beads followed by pepsin digestion and chromatographic separation.  Coupling to R-PE was followed by size exclusion chromatography to purify conjugate from unreacted R-PE and antibody.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Goat Serum, Rabbit IgG and Rabbit Serum.  No reaction was observed against anti-Pepsin or anti-Goat IgG F(c).
Storage Condition Store vial at 4° C prior to opening. Dilute only prior to immediate use. Do not freeze after reconstitution. Store reagent in the dark. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis.
Precautions NoteThis product is for research use only and is not intended for therapeutic or diagnostic applications.
Research Areas
Citations (0)
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$ 252.00
Cat# ASR2871
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Availability: 3-5 days
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