F(ab')2 Anti-Rabbit IgG (H&L) (Phycoerythrin Conjugated) Secondary Antibody
Goat Polyclonal, R-Phycoerythrin (RPE)
- SPECIFICATION
- CITATIONS
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- BACKGROUND
Description | F(ab')2 Anti-RABBIT IgG [H&L] (GOAT) Antibody Phycoerythrin conjugated |
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Host | Goat |
Conjugate | R-Phycoerythrin (RPE) |
Target Species | Rabbit |
Clonality | Polyclonal |
Application
| IF, FC |
Application Note | IF Microscopy 1:100-1:250 FlowCytometry 1:100-1:250 FLOW1:100-1:250 |
Physical State | Lyophilized |
Host Isotype | IgG F(ab')2 |
Target Isotype | IgG (H&L) |
Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
Immunogen | Rabbit IgG whole molecule |
Reconstitution Volume | 1.0 mL |
Reconstitution Buffer | Restore with deionized water (or equivalent) |
Stabilizer | 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free |
Preservative | 0.01% (w/v) Sodium Azide |
Shipping Condition | Ambient |
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Purity | This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by pepsin digestion and chromatographic separation. Coupling to R-PE was followed by size exclusion chromatography to purify conjugate from unreacted R-PE and antibody. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Goat Serum, Rabbit IgG and Rabbit Serum. No reaction was observed against anti-Pepsin or anti-Goat IgG F(c). |
Storage Condition | Store vial at 4° C prior to opening. Dilute only prior to immediate use. Do not freeze after reconstitution. Store reagent in the dark. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis. |
Precautions Note | This product is for research use only and is not intended for therapeutic or diagnostic applications. |
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