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Anti-Mouse IgG (H&L) (Biotin Conjugated) Pre-Adsorbed Secondary Antibody
Donkey Polyclonal, Biotin
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
| Description | Anti-MOUSE IgG (H&L) (DONKEY) Antibody Biotin Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Rb Rt & Sh Serum Proteins) |
|---|---|
| Host | Donkey |
| Conjugate | Biotin |
| Target Species | Mouse |
| Reactivity | Mouse |
| Clonality | Polyclonal |
Application 
| WB, E, IC |
| Application Note | ELISA 1:20,000-1:100,000 Western Blot 1:2,000-1:10,000 Immunochemistry 1:1,000-1:5,000 |
| Physical State | Lyophilized |
| Host Isotype | IgG |
| Target Isotype | IgG (H&L) |
| Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Immunogen | Mouse IgG whole molecule |
| Reconstitution Volume | 1.0 mL |
| Reconstitution Buffer | Restore with deionized water (or equivalent) |
| Stabilizer | 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free |
| Preservative | 0.01% (w/v) Sodium Azide |
| Shipping Condition | Ambient |
|---|---|
| Purity | Anti-Mouse IgG antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Donkey Serum, Mouse IgG and Mouse Serum. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Rabbit, Rat and Sheep Serum Proteins. |
| Storage Condition | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Precautions Note | This product is for research use only and is not intended for therapeutic or diagnostic applications. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Mouse IgG antibody generated in donkey detects specifically Mouse IgG whole molecule. This secondary antibody anti-Mouse is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays.
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