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Anti-Mouse IgG (gamma 1, 2a, 2b and 3 chain) (Rhodamine Conjugated) Secondary Antibody
Rabbit Polyclonal, Rhodamine (TRITC)
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| Description | Anti-MOUSE IgG (gamma 1, 2a, 2b and 3 chain) (RABBIT) Antibody Rhodamine Conjugated |
|---|---|
| Host | Rabbit |
| Conjugate | Rhodamine (TRITC) |
| FP Value | 3.1 moles Rhodamine (TRITC) per mole of IgG |
| Target Species | Mouse |
| Clonality | Polyclonal |
Application 
| WB, IF, FC, IC |
| Application Note | FLISA 1:20,000-1:100,000 IF Microscopy 1:1,000-1:5,000 FlowCytometry 1:1,000-1:5,000 Western Blot 1:2,000-1:10,000 Immunochemistry 1:1,000-1:5,000 |
| Physical State | Lyophilized |
| Host Isotype | IgG |
| Target Isotype | IgG (gamma 1, 2a, 2b and 3 chain) |
| Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Immunogen | highly purified mouse IgG gamma 1, gamma 2a, gamma 2b and gamma 3 proteins |
| Reconstitution Volume | 1.0 mL |
| Reconstitution Buffer | Restore with deionized water (or equivalent) |
| Stabilizer | 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free |
| Preservative | 0.01% (w/v) Sodium Azide |
| Shipping Condition | Ambient |
|---|---|
| Purity | Anti-Mouse IgG subclass pan reactive Secondary Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. This product shows balanced reactivity to Mouse IgG1, IgG2a, IgG2b and IgG3 proteins and is suitable to screen IgG class hybridoma clones. Minimal cross reactivity is observed against other Mouse immunoglobulin classes or light chain proteins. |
| Storage Condition | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Precautions Note | This product is for research use only and is not intended for therapeutic or diagnostic applications. |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
Rhodamine Conjugated Secondary Antibodies are ideal for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting and are available in a variety of formats and conjugate types. When choosing a secondary antibody, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
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