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Anti-Human IgG F(c) (Alkaline Phosphatase Conjugated) Secondary Antibody
Rabbit Polyclonal, Alkaline Phosphatase (Calf Intestine)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
| Description | Anti-HUMAN IgG F(c) (RABBIT) Antibody Alkaline Phosphatase Conjugated |
|---|---|
| Host | Rabbit |
| Conjugate | Alkaline Phosphatase (Calf Intestine) |
| Target Species | Human |
| Reactivity | Human |
| Clonality | Polyclonal |
Application 
| WB, IC, E |
| Application Note | ELISA 1:2,000-1:10,000 Western Blot 1:500-1:2,500 Immunochemistry 1:200-1:1,000 |
| Physical State | Liquid (sterile filtered) |
| Host Isotype | IgG |
| Target Isotype | IgG F(c) |
| Buffer | 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0 |
| Immunogen | Anti-Human IgG F(c) was produced by repeated immunization with Human Fc fragment in rabbit. |
| Stabilizer | 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free |
| Preservative | 0.1% (w/v) Sodium Azide |
| Shipping Condition | Wet Ice |
|---|---|
| Purity | Anti-Human IgG F(c) was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Human IgG, Human IgG F(c) and Human Serum. No reaction was observed against Human IgG F(ab’)2. |
| Storage Condition | Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. |
| Precautions Note | This product is for research use only and is not intended for therapeutic or diagnostic applications. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Human IgG F(c) antibody generated in rabbit detects specifically Anti-Human IgG F(c) fragment. This secondary antibody anti-Human IgG F(c) is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays.
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