Anti-Human IgG (H&L) (Rhodamine Conjugated) Secondary Antibody
Mouse Polyclonal, Rhodamine (TRITC)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Description | Anti-HUMAN IgG (H&L) (MOUSE) Antibody Rhodamine Conjugated |
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Host | Mouse |
Conjugate | Rhodamine (TRITC) |
FP Value | 2.1 moles Rhodamine (TRITC) per mole of IgG |
Target Species | Human |
Reactivity | Human |
Clonality | Polyclonal |
Application
| IF |
Application Note | IF Microscopy 1:500-1:2,500 |
Physical State | Lyophilized |
Host Isotype | IgG |
Target Isotype | IgG (H&L) |
Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
Immunogen | Human IgG whole molecule |
Reconstitution Volume | 1.0 mL |
Reconstitution Buffer | Restore with deionized water (or equivalent) |
Stabilizer | 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free |
Preservative | 0.01% (w/v) Sodium Azide |
Shipping Condition | Ambient |
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Purity | Human IgG (H&L) Antibody Rhodamine Conjugated was prepared from monospecific polyclonal ascites by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse Serum, Human IgG and Human Serum. |
Storage Condition | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
Precautions Note | This product is for research use only and is not intended for therapeutic or diagnostic applications. |
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