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>   home   >   Products   >   Secondary Antibodies   >   Rhodamine (TRITC)   >   F(ab')2 Anti-Rat IgG F(c) (Biotin Conjugated) Pre-Adsorbed Secondary Antibody   

F(ab')2 Anti-Rat IgG F(c) (Biotin Conjugated) Pre-Adsorbed Secondary Antibody

Goat Polyclonal, Biotin

     
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Product Information
Description F(ab')2 Anti-RAT IgG F(c) (GOAT) Antibody Biotin Conjugated Min X Bv Hs & Hu Serum Proteins
Host Goat
Conjugate Biotin
Target Species Rat
Clonality Polyclonal
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, E, IC
Application Note ELISA 1:20,000-1:100,000
Western Blot 1:2,000-1:10,000
Immunochemistry 1:1,000-1:5,000
Physical State Lyophilized
Host Isotype IgG F(ab')2
Target Isotype IgG F(c)
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Immunogen Rat IgG F(c) fragment
Reconstitution Volume 500 µL
Reconstitution Buffer Restore with deionized water (or equivalent)
Stabilizer 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative 0.01% (w/v) Sodium Azide
Additional Information
Shipping Condition Ambient
Purity This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum, Rat IgG, Rat IgG F(c) and Rat Serum.  No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Rat IgG F(ab’)2 or Bovine, Horse and Human Serum Proteins.
Storage Condition Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Precautions NoteThis product is for research use only and is not intended for therapeutic or diagnostic applications.
Research Areas
Citations (0)
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Background

Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.

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$ 220.00
Cat# ASR2639
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Availability: 3-5 days
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