Anti-Human IgG IgA IgM (H&L) (Peroxidase Conjugated) Secondary Antibody
Goat Polyclonal, Peroxidase (Horseradish)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Description | Anti-HUMAN IgG IgA IgM (H&L) (GOAT) Antibody Peroxidase Conjugated |
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Host | Goat |
Conjugate | Peroxidase (Horseradish) |
Target Species | Human |
Clonality | Polyclonal |
Application
| WB, IP, E, IC |
Application Note | ELISA 1:20,000-1:100,000 Western Blot 1:2,000-1:10,000 Immunochemistry 1:1,000-1:5,000ImmunoPrecipitation:1:1,000-1:5,000 |
Physical State | Lyophilized |
Host Isotype | IgG |
Target Isotype | IgG IgA IgM |
Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
Immunogen | Anti-Human IgG IgA IgM (H&L) was produced by repeated immunization with Human IgG, IgA and IgM whole molecules in goat. |
Reconstitution Volume | 1.0 mL |
Reconstitution Buffer | Restore with deionized water (or equivalent) |
Stabilizer | 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free |
Preservative | 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! |
Shipping Condition | Ambient |
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Purity | This product was prepared from polyspecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Human IgG, Human IgA and Human IgM. This reagent is suitable for the detection of all Human immunoglobulin subclasses and chain combinations. |
Storage Condition | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
Precautions Note | This product is for research use only and is not intended for therapeutic or diagnostic applications. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Anti-Human IgG IgA IgM (H&L) antibody generated in goat detects specifically human IgG IgA IgM (H&L). This secondary antibody anti-Human is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays.
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