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Anti-Monkey IgG (gamma chain) (Rhodamine Conjugated) Secondary Antibody

Goat Polyclonal, Rhodamine (TRITC)

     
  • WB - Anti-Monkey IgG (gamma chain)  (Rhodamine Conjugated) Secondary Antibody ASR2236
    Western Blot showing detection of Monkey IgG Gamma Chain. 100ng of Monkey IgG (Lane 2) was run on a 4-20% gel and transferred to 0.45 µm nitrocellulose. After blocking with 5% Blotto 30 min at 20°C, Anti-Monkey IgG (gamma chain) (GOAT) Antibody Rhodamine Conjugated secondary antibody was used at 1:1000 in Blocking Buffer for Fluorescent Western Blotting and imaged using the Bio-Rad VersaDoc® 4000 MP. Molecular weight markers are in lane 1.
    detail
  •  - Anti-Monkey IgG (gamma chain)  (Rhodamine Conjugated) Secondary Antibody ASR2236
    This table displays additional reactivity among various species of serum and immunoglobulin. A (+) indicates antibody reactivity to the corresponding target.
    detail
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Product Information
Description Anti-MONKEY IgG (gamma chain) (GOAT) Antibody Rhodamine Conjugated
Host Goat
Conjugate Rhodamine (TRITC)
FP Value 2.0 moles Rhodamine (TRITC) per mole of IgG
Target Species Monkey
Clonality Polyclonal
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
IF, FC
Application Note FLISA 1:10,000-1:50,000
IF Microscopy 1:1,000-1:5,000
FlowCytometry 1:500-1:2,500
Physical State Lyophilized
Host Isotype IgG
Target Isotype IgG (gamma chain)
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Immunogen Monkey IgG gamma heavy chain
Reconstitution Volume 1.0 mL
Reconstitution Buffer Restore with deionized water (or equivalent)
Stabilizer 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative 0.01% (w/v) Sodium Azide
Additional Information
Shipping Condition Ambient
Purity This product was prepared from monospecific antiserum by immunoaffinity chromatography using Monkey IgA coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Monkey IgG and Monkey Serum.   No reaction was observed against Monkey gA or Monkey IgM.   Specificity was confirmed by ELISA at less than 1% cross reactivity against other Monkey heavy or light chain isotypes.
Storage Condition Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Precautions NoteThis product is for research use only and is not intended for therapeutic or diagnostic applications.
Research Areas
Citations (0)
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Background

This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

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$ 199.00
Cat# ASR2236
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