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Anti-Mouse IgG (H&L) (Peroxidase Conjugated) Secondary Antibody

Donkey Polyclonal, Peroxidase (Horseradish)

     
  • WB - Anti-Mouse IgG (H&L)  (Peroxidase Conjugated) Secondary Antibody ASR1937
    Western Blot of Donkey anti-Mouse IgG (H&L) Antibody Peroxidase Conjugated. Lane 1: Mouse IgG. Lane 2: None. Load: 50 ng per lane. Primary Antibody: None. Secondary antibody: Mouse IgG (H&L) Antibody Peroxidase Conjugated at 1:1,000 for 60 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 55 and 28 kDa, 55 and 28 kDa for Mouse IgG. Other Band(s): None.
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  • SPECIFICATION
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Product Information
Description Anti-MOUSE IgG (H&L) (DONKEY) Antibody Peroxidase Conjugated
Host Donkey
Conjugate Peroxidase (Horseradish)
Target Species Mouse
Reactivity Mouse
Clonality Polyclonal
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, E, IC
Application Note ELISA 1:70,000
Western Blot 1:2,000to1:10,000
Immunochemistry 1:500-1:2,500
Physical State Lyophilized
Host Isotype IgG
Target Isotype IgG (H&L)
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Immunogen Anti-Mouse IgG was produced by repeated immunization with Mouse IgG whole molecule in donkey
Reconstitution Volume 1.0 mL
Reconstitution Buffer Restore with deionized water (or equivalent)
Stabilizer 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
Additional Information
Shipping Condition Ambient
Purity This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Donkey Serum, Mouse IgG and Mouse Serum.
Storage Condition Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Precautions NoteThis product is for research use only and is not intended for therapeutic or diagnostic applications.
Research Areas
Citations ( 0 )

Background

Anti-Mouse IgG (H&L) peroxidase conjugated antibody generated in donkey detects specifically mouse IgG (H&L). This secondary peroxidase conjugated antibody anti-Mouse is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.

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$ 163.00
Cat# ASR1937
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Availability: 3-5 days
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