Anti-Mouse IgG (H&L) (Peroxidase Conjugated) Secondary Antibody
Donkey Polyclonal, Peroxidase (Horseradish)
- SPECIFICATION
- CITATIONS: 1
- PROTOCOLS
- BACKGROUND
Description | Anti-MOUSE IgG (H&L) (DONKEY) Antibody Peroxidase Conjugated |
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Host | Donkey |
Conjugate | Peroxidase (Horseradish) |
Target Species | Mouse |
Reactivity | Mouse |
Clonality | Polyclonal |
Application
| WB, E, IC |
Application Note | ELISA 1:70,000 Western Blot 1:2,000to1:10,000 Immunochemistry 1:500-1:2,500 |
Physical State | Lyophilized |
Host Isotype | IgG |
Target Isotype | IgG (H&L) |
Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
Immunogen | Anti-Mouse IgG was produced by repeated immunization with Mouse IgG whole molecule in donkey |
Reconstitution Volume | 1.0 mL |
Reconstitution Buffer | Restore with deionized water (or equivalent) |
Stabilizer | 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free |
Preservative | 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! |
Shipping Condition | Ambient |
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Purity | This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Donkey Serum, Mouse IgG and Mouse Serum. |
Storage Condition | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
Precautions Note | This product is for research use only and is not intended for therapeutic or diagnostic applications. |
Provided below are standard protocols that you may find useful for product applications.
Background
Anti-Mouse IgG (H&L) peroxidase conjugated antibody generated in donkey detects specifically mouse IgG (H&L). This secondary peroxidase conjugated antibody anti-Mouse is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.
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