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Anti-Rat IgG (H&L) (Biotin Conjugated) Secondary Antibody
Goat Polyclonal, Biotin
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
| Description | Anti-RAT IgG (H&L) (GOAT) Antibody Biotin Conjugated |
|---|---|
| Host | Goat |
| Conjugate | Biotin |
| Target Species | Rat |
| Clonality | Polyclonal |
Application 
| WB, E, IC |
| Application Note | ELISA 1:20,000-1:100,000 Western Blot 1:2,000-1:10,000 Immunochemistry 1:1,000-1:5,000 |
| Physical State | Lyophilized |
| Host Isotype | IgG |
| Target Isotype | IgG (H&L) |
| Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Immunogen | Rat IgG whole molecule |
| Reconstitution Volume | 1.0 mL |
| Reconstitution Buffer | Restore with deionized water (or equivalent) |
| Stabilizer | 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free |
| Preservative | 0.01% (w/v) Sodium Azide |
| Shipping Condition | Ambient |
|---|---|
| Purity | Anti-RAT IgG Biotin Conjugated antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Goat Serum, Rat IgG and Rat Serum. |
| Storage Condition | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Precautions Note | This product is for research use only and is not intended for therapeutic or diagnostic applications. |
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Provided below are standard protocols that you may find useful for product applications.
Background
RAT IgG Biotin Conjugated antibody detects rat immunoglobulin G. Immunoglobuoin G is a molecule of about 150 kDa composed of four peptide chains. Each IgG contains two identical ? heavy chains of about 51 kDa and two identical light chains of about 26 kDa, thus a tetrameric quaternary structure. The two heavy chains are linked to each other and to a light chain each by disulfide bonds. The resulting tetramer has two identical halves, which together form the Y-like shape. Each end of the fork contains an identical antigen binding site. The Fc regions of IgGs bear a highly conserved N-glycosylation site.
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