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Anti-Mouse IgG IgA IgM (H&L) Pre-Adsorbed Secondary Antibody

     
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Product Information
Description Anti-MOUSE IgG IgA IgM (H&L) (GOAT) (Min X Bv, Hs, Hu Serum Proteins)
Host Goat
Conjugate Unconjugated
Target Species Mouse
Reactivity Mouse
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, E, IC
Application Note ELISA 1:200,000
Western Blot 1:2,000-1:10,000
Immunochemistry 1:1,000-1:5,000
Physical State Liquid (sterile filtered)
Host Isotype IgG
Target Isotype IgG IgA IgM
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Immunogen Mouse IgG IgA and IgM whole molecule
Stabilizer None
Preservative 0.01% (w/v) Sodium Azide
Additional Information
Shipping Condition Wet Ice
Purity Secondary Antibody was prepared from polyspecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by cross adsorption to remove unwanted specificities.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse IgG, Mouse IgA and Mouse IgM.  No reaction was observed against bovine, horse or human serum proteins.  This reagent is suitable for the detection of all mouse isotypes and chain combinations.  ELISA was used to confirm specificity at less than 1% of the target signal.
Storage Condition Store vial at 4° C prior to opening.  For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.
Precautions NoteThis product is for research use only and is not intended for therapeutic or diagnostic applications.
Research Areas
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$ 169.00
Cat# ASR1234
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