Anti-Mouse IgG IgA IgM (H&L) Pre-Adsorbed Secondary Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Description | Anti-MOUSE IgG IgA IgM (H&L) (GOAT) (Min X Bv, Hs, Hu Serum Proteins) |
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Host | Goat |
Conjugate | Unconjugated |
Target Species | Mouse |
Reactivity | Mouse |
Application
| WB, E, IC |
Application Note | ELISA 1:200,000 Western Blot 1:2,000-1:10,000 Immunochemistry 1:1,000-1:5,000 |
Physical State | Liquid (sterile filtered) |
Host Isotype | IgG |
Target Isotype | IgG IgA IgM |
Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
Immunogen | Mouse IgG IgA and IgM whole molecule |
Stabilizer | None |
Preservative | 0.01% (w/v) Sodium Azide |
Shipping Condition | Wet Ice |
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Purity | Secondary Antibody was prepared from polyspecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by cross adsorption to remove unwanted specificities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse IgG, Mouse IgA and Mouse IgM. No reaction was observed against bovine, horse or human serum proteins. This reagent is suitable for the detection of all mouse isotypes and chain combinations. ELISA was used to confirm specificity at less than 1% of the target signal. |
Storage Condition | Store vial at 4° C prior to opening. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
Precautions Note | This product is for research use only and is not intended for therapeutic or diagnostic applications. |
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