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Anti-Mouse IgM (mu chain) Secondary Antibody
Rabbit Polyclonal, Unconjugated
- SPECIFICATION
- CITATIONS
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- BACKGROUND
| Description | Anti-MOUSE IgM (mu chain) (RABBIT) Antibody |
|---|---|
| Host | Rabbit |
| Conjugate | Unconjugated |
| Target Species | Mouse |
| Reactivity | Mouse |
| Clonality | Polyclonal |
Application 
| WB, E, IC |
| Application Note | ELISA 1:20,000-1:100,000 Western Blot 1:2,000-1:10,000 Immunochemistry 1:500-1:2,500 |
| Physical State | Liquid (sterile filtered) |
| Host Isotype | IgG |
| Target Isotype | IgM µ chain |
| Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Immunogen | Anti-Mouse IgM mu heavy chain antibody was produced by repeated immunization with Mouse IgM mu heavy chain in rabbit. |
| Stabilizer | None |
| Preservative | 0.01% (w/v) Sodium Azide |
| Shipping Condition | Wet Ice |
|---|---|
| Purity | This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Mouse IgM and Mouse Serum. No reaction was observed against other mouse or human heavy or light chain proteins. |
| Storage Condition | Store vial at 4° C prior to opening. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. |
| Precautions Note | This product is for research use only and is not intended for therapeutic or diagnostic applications. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Anti-Mouse IgM mu heavy chain antibody generated in rabbit detects specifically Mouse IgM mu heavy chain. This secondary antibody anti-Mouse is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.
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