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VPS35 Antibody

VPS35 Antibody, Clone 8A3

     
  • IHC - VPS35 Antibody ASM10693
    Immunohistochemistry analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 8A3 (ASM10693). Tissue: Kidney. Species: Mouse. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody (ASM10693) at 1:100 for Overnight at 4C, then 30 min at 37C. Secondary Antibody: Goat Anti-Mouse IgG (H+L): FITC for 45 min at 37C. Counterstain: DAPI for 3 min at RT. Magnification: 20X.
    detail
  •  - VPS35 Antibody ASM10693
    Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 8A3 (ASM10693). Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody (ASM10693) at 1:5 (tissue culture supernatant). Secondary Antibody: Donkey anti-mouse: Alexa Fluor 594 at 1:4000 in 0.2% BSA PBS. Counterstain: DAPI. Localization: Vesicles. A) VPS35 KO A549 cells B) WT A549 cells. Courtesy of: Dario Alessi Lab, University of Dundee.
    detail
  •  - VPS35 Antibody ASM10693
    Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 8A3 (ASM10693). Tissue: A549 WT, VPS35 KO cells. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody (ASM10693). Secondary Antibody: Donkey Anti-Mouse AlexaFluor 594. Clone can detect VPS35 at 1/2000 concentration.
    detail
  • IP - VPS35 Antibody ASM10693
    Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 8A3 (ASM10693). Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody (ASM10693). 500 µL cell culture supernatants were incubated with 10 µL of Protein A/G resin beads for 1 hour at 4⁰C. ASM10693 clone 8A3 depletes VPS35 from the A549 cell extract..
    detail
  • IP - VPS35 Antibody ASM10693
    Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 8A3 (ASM10693). Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody (ASM10693).
    detail
  • WB - VPS35 Antibody ASM10693
    Western Blot analysis of Human, Mouse A549, MEF showing detection of VPS35 protein using Mouse Anti-VPS35 Monoclonal Antibody, Clone 8A3 (ASM10693). Lane 1: Molecular Weight Ladder. Lane 2: VPS35 KO A549 cells. Lane 3: mouse embryonic fibroblast cells.. Load: 8 µg each A549 and MEF. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody (ASM10693) at 1:5 (tissue culture supernatant). Secondary Antibody: Donkey anti-mouse IRDye 800CW at 1:25000 in TBS-T.
    detail
  • WB - VPS35 Antibody ASM10693
    Western Blot analysis of Human SH-SY5Y showing detection of VPS35 protein using Mouse Anti-VPS35 Monoclonal Antibody, Clone 8A3 (ASM10693). Lane 1: Molecular Weight Ladder. Lane 2: SH-SY5Y (10 ug). Load: 10 µg. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody (ASM10693) at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT.
    detail
  • IP - VPS35 Antibody ASM10693
    Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 8A3 (ASM10693). Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody (ASM10693). Three amounts of ASM10693 (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4°C and next incubated with 250ug of A549 lysate for 2 hours at 4°C.
    detail
  • IP - VPS35 Antibody ASM10693
    Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 8A3 (ASM10693). Tissue: embryonic fibroblast. Species: Mouse. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody (ASM10693). Three amounts of ASM10693 (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4°C and next incubated with 250ug of MEF lysate for 2 hours at 4°C.
    detail
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Product Information
Primary Accession Q96QK1
Other Accession NP_060676.2
Host Mouse
Clonality Monoclonal
Target/Specificity VPS35
Other Names Vacuolar protein sorting-associated protein 35, MEM3, PARK17, VPS35 retromer complex component, maternal-embryonic 3, vesicle protein sortin 35, TCCCTA00141, FLJ10752
Clone Names VPS35
Immunogen Full length recombinant human VSP35
Purification Protein G Purified
Storage -20ºC
Storage Buffer PBS pH 7.4, 50% glycerol, 0.09% Sodium azide *Storage buffer may change when conjugated
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis A 1:1000 dilution of SMC-604 was sufficient for detection of VPS35 in 10 µg of SH-SY5Y by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary antibody.
Cellular Localization Endosome | Lysosome | Vesicles | Cytoplasm | Membrane
Research Areas
Citations (0)
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Background

Vacuolar Protein Sorter-35 (VPS35) is a component of the retromer complex, which is essential for endosome-to-Golgi retrieval of membrane proteins. VPS35 mutations such as D620N have been linked to Parkinson’s Disease (PD) (1,2) and affect retromer function, protein homeostasis, and mitochondria (3).

References

1. Vilarino-Guell, C. et al. (2011) Am J Hum Genet 89:162–167
2. Zimprich, A. et al. (2011) Am J Hum Genet 89:168–175
3. Rahman, A.A., Morrison, B.E. (2019) Neurosci 401:1-10.

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$ 395.00
Cat# ASM10693
11 Formats Available
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