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VPS35 Antibody
VPS35 Antibody, Clone 8A3
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
| Primary Accession | Q96QK1 |
|---|---|
| Other Accession | NP_060676.2 |
| Host | Mouse |
| Clonality | Monoclonal |
| Target/Specificity | VPS35 |
| Other Names | Vacuolar protein sorting-associated protein 35, MEM3, PARK17, VPS35 retromer complex component, maternal-embryonic 3, vesicle protein sortin 35, TCCCTA00141, FLJ10752 |
| Clone Names | VPS35 |
| Immunogen | Full length recombinant human VSP35 |
| Purification | Protein G Purified |
| Storage | -20ºC |
| Storage Buffer | PBS pH 7.4, 50% glycerol, 0.09% Sodium azide *Storage buffer may change when conjugated |
| Shipping Temperature | Blue Ice or 4ºC |
| Certificate of Analysis | A 1:1000 dilution of SMC-604 was sufficient for detection of VPS35 in 10 µg of SH-SY5Y by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary antibody. |
| Cellular Localization | Endosome | Lysosome | Vesicles | Cytoplasm | Membrane |
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Provided below are standard protocols that you may find useful for product applications.
Background
Vacuolar Protein Sorter-35 (VPS35) is a component of the retromer complex, which is essential for endosome-to-Golgi retrieval of membrane proteins. VPS35 mutations such as D620N have been linked to Parkinson’s Disease (PD) (1,2) and affect retromer function, protein homeostasis, and mitochondria (3).
References
1. Vilarino-Guell, C. et al. (2011) Am J Hum Genet 89:162–167
2. Zimprich, A. et al. (2011) Am J Hum Genet 89:168–175
3. Rahman, A.A., Morrison, B.E. (2019) Neurosci 401:1-10.
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