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Ubiquitin Antibody

Ubiquitin Antibody, Clone FK1

     
  •  - Ubiquitin Antibody ASM10654
    Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Ubiquitin Monoclonal Antibody, Clone FK1 (ASM10654). Tissue: Fibroblast cell line (NIH 3T3). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Ubiquitin Monoclonal Antibody (ASM10654) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Nucleus, Cytoplasm. Magnification: 60X.
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  • WB - Ubiquitin Antibody ASM10654
    Western Blot analysis of Poly-Ubiquitin showing detection of Ubiquitin protein using Mouse Anti-Ubiquitin Monoclonal Antibody, Clone FK1 (ASM10654). Lane 1: MW ladder. Lane 2: Poly-Ubiquitin, K48-linked (2 ug). Lane 2: Poly-Ubiquitin, K63-linked (0.6 ug). Load: 5 uL. Block: 1% BSA in TBST. Primary Antibody: Mouse Anti-Ubiquitin Monoclonal Antibody (ASM10654) at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:2000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT.
    detail
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Product Information
Primary Accession P0CG47
Other Accession BAC56955.1
Host Mouse
Clonality Monoclonal
Target/Specificity Ubiquitin
Other Names Polyubiquitin B Antibody, RPS27A Antibody, UBA52 Antibody, UBB Antibody, UBC Antibody, ubiquitin B Antibody
Clone Names Ubiquitin
Immunogen Human Ubiquitin conjugated lysozyme
Purification Gel filtration
Storage -20ºC
Storage Buffer 10mM phosphate buffer, 0.15M NaCl pH7.4, 0.1% sodium azide *Storage buffer may change when conjugated
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis A 1:5000 dilution of SMC-213 was sufficient for detection of ubiquitin conjugates in 2.5 µg of HeLa cell lysate by electrochemiluminescence analysis using goat anti-mouse IgG:HRP as the secondary antibody.
Cellular Localization Cytoplasm | Nucleus
Research Areas
Citations (0)
citation

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Background

Ubiquitin is a small protein found ubiquitously in all tissue types and acts as a post translational modification. It can bind to its substrate either as a single ubiquitin molecule or in a chain. It is involved in many regulatory processes, which include proteasomal degradation, signal transduction, DNA repair, endocytosis and autophagy.

References

1. Chen J., & Chen Z. (2013). Curr Opin Immunol. (1): 4-12.
2. Shaid S., Brandts C., Serve H., & Dikic I. (2013). Cell Death Differ. 20(1): 21-30.

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$ 45.00
Cat# ASM10654
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