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NRAS Antibody

Rabbit Anti-Human NRAS Polyclonal

     
  •  - NRAS Antibody ASM10645
    Western blot analysis of Rat Kidney showing detection of 21.2 kDa NRAS protein using Rabbit Anti-NRAS Polyclonal Antibody (SPC-796). Load: 15 µg. Block: 5% Skim Milk powder in TBST. Primary Antibody: Rabbit Anti-NRAS Polyclonal Antibody (SPC-796) at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: 21.2 kDa. Other Band(s): 25 kDa.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession P01111
Other Accession NP_002515.1
Host Rabbit
Reactivity Rat
Clonality Polyclonal
Format NRAS
Target/Specificity NRAS
Other Names Transforming protein N-Ras Antibody, GTPase NRas Antibody, NRAS Antibody, HRAS1 Antibody
Clone Names NRAS
Immunogen Synthetic peptide from the C-terminal of Human GTPase NRas (aa. 85-185)
Purification Peptide Affinity Purified
Storage -20ºC
Storage Buffer PBS pH 7.4, 50% glycerol, 0.09% sodium azide *Storage buffer may change when conjugated
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis A 1:1000 dilution of SPC-796 was sufficient for detection of NRAS in 15 µg of rat Kidney cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Cellular Localization Cell Membrane | Lipid-Anchor | Cytoplasmic Side | Golgi Apparatus Membrane | Lipid-Anchor
Research Areas
Citations (0)
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$ 330.00
Cat# ASM10645
11 Formats Available
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