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AKT Antibody

Rabbit Anti-Human AKT1 Polyclonal

     
  •  - AKT Antibody ASM10638
    Immunohistochemistry analysis using Rabbit Anti-AKT Polyclonal Antibody (SPC-789). Tissue: Breast Cancer. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-AKT Polyclonal Antibody (SPC-789) at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 10X. HRP-DAB Detection. | Western blot analysis of Rat Liver showing detection of 55.7 kDa AKT1 protein using Rabbit Anti-AKT1 Polyclonal Antibody (SPC-789). Load: 15 µg. Block: 5% Skim Milk powder in TBST. Primary Antibody: Rabbit Anti-AKT1 Polyclonal Antibody (SPC-789) at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: 55.7 kDa.
    detail
  •  - AKT Antibody ASM10638
    Immunohistochemistry analysis using Rabbit Anti-AKT Polyclonal Antibody (SPC-789). Tissue: Breast Cancer. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-AKT Polyclonal Antibody (SPC-789) at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 10X. HRP-DAB Detection. | Western blot analysis of Rat Liver showing detection of 55.7 kDa AKT1 protein using Rabbit Anti-AKT1 Polyclonal Antibody (SPC-789). Load: 15 µg. Block: 5% Skim Milk powder in TBST. Primary Antibody: Rabbit Anti-AKT1 Polyclonal Antibody (SPC-789) at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: 55.7 kDa.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
IHC
Primary Accession P31749
Other Accession NP_001014431.1
Host Rabbit
Reactivity Rat
Clonality Polyclonal
Format AKT1
Target/Specificity AKT1
Other Names RAC-alpha serine/threonine-protein kinase Antibody, Protein Kinase B Alpha Antibody, EC:2.7.11.1 Antibody, AKT1 Antibody, AKT Antibody, MGC99656 Antibody, PKB alpha Antibody, PKB-ALPHA Antibody, AKT 1 Antibody, RAC-PK-alpha Antibody, PRKBA Antibody, PKB Antibody, Protein kinase B alpha Antibody, Protein kinase B Antibody, Proto-oncogene c-Akt Antibody, AKT1_HUMAN Antibody, RAC Antibody, RAC Alpha Antibody
Clone Names AKT1
Immunogen Synthetic peptide from the N-terminal of Human RAC-alpha serine/threonine-protein kinase (aa. 1-100)
Purification Peptide Affinity Purified
Storage -20ºC
Storage Buffer PBS pH 7.4, 50% glycerol, 0.09% sodium azide *Storage buffer may change when conjugated
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis A 1:1000 dilution of SPC-789 was sufficient for detection of AKT1 in 15 µg of rat Liver cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Cellular Localization Cytoplasm | Nucleus | Cell Membrane
Research Areas
Citations (0)
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$ 330.00
Cat# ASM10638
11 Formats Available
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