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SMAD3 Antibody

Rabbit Anti-Human SMAD3 Polyclonal

     
  •  - SMAD3 Antibody ASM10603
    Immunohistochemistry analysis using Rabbit Anti-SMAD3 Polyclonal Antibody (SPC-754). Tissue: Colon Cancer. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-SMAD3 Polyclonal Antibody (SPC-754) at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 10X. HRP-DAB Detection. | Western blot analysis of Human Lung carcinoma epithelial cell line (A549) lysate showing detection of ~48.1 kDa SMAD3 protein using Rabbit Anti-SMAD3 Polyclonal Antibody (SPC-754). Lane 1: Molecular Weight Ladder (MW). Lane 2: A549. Load: 10 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-SMAD3 Polyclonal Antibody (SPC-754) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~48.1 kDa.
    detail
  •  - SMAD3 Antibody ASM10603
    Immunohistochemistry analysis using Rabbit Anti-SMAD3 Polyclonal Antibody (SPC-754). Tissue: Colon Cancer. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-SMAD3 Polyclonal Antibody (SPC-754) at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 10X. HRP-DAB Detection. | Western blot analysis of Human Lung carcinoma epithelial cell line (A549) lysate showing detection of ~48.1 kDa SMAD3 protein using Rabbit Anti-SMAD3 Polyclonal Antibody (SPC-754). Lane 1: Molecular Weight Ladder (MW). Lane 2: A549. Load: 10 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-SMAD3 Polyclonal Antibody (SPC-754) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~48.1 kDa.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
IHC
Primary Accession P84022
Other Accession NP_001138574.1
Host Rabbit
Clonality Polyclonal
Format SMAD3
Target/Specificity SMAD3
Other Names hSMAD3 Antibody, DKFZp686J10186 Antibody, Mothers against decapentaplegic homolog 3 Antibody, Mothers against DPP homolog 3 Antibody, hMAD 3 Antibody, DKFZP586N0721 Antibody, Mad homolog JV15 2 Antibody, LDS1C Antibody, HSPC193 Antibody, MAD homolog 3 Antibody, SMAD3 Antibody, MGC60396 Antibody, Mad3 Antibody, SMAD family member 3 Antibody, SMAD 3 Antibody, MAD (mothers against decapentaplegic Drosophila) homolog 3 Antibody, JV15 2 Antibody, SMAD3_HUMAN Antibody, MADH3 Antibody, JV15-2 Antibody, MAD, mothers against decapentaplegic homolog 3 Antibody, HST17436 Antibody, Smad3 Antibody, SMA and MAD related protein 3 Antibody, SMAD, mothers against DPP homolog 3 Antibody, MADH 3 Antibody, LDS3 Antibody, SMAD Antibody, JV152 Antibody, Mad protein homolog Antibody, hMAD-3 Antibody
Clone Names SMAD3
Immunogen Synthetic peptide from the N-terminal of Human SMAD3 (aa. 1-100)
Purification Peptide Affinity Purified
Storage -20ºC
Storage Buffer PBS pH 7.4, 50% glycerol, 0.09% sodium azide *Storage buffer may change when conjugated
Shipping Temperature Blue Ice or 4ºC
Cellular Localization Cytoplasm | Nucleus
Research Areas
Citations (0)
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$ 330.00
Cat# ASM10603
11 Formats Available
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