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RYK Antibody

Rabbit Anti-Human RYK Polyclonal

     
  •  - RYK Antibody ASM10589
    Immunohistochemistry analysis using Rabbit Anti-RYK Polyclonal Antibody (SPC-740). Tissue: Breast Cancer. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-RYK Polyclonal Antibody (SPC-740) at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 10X. HRP-DAB Detection. | Western blot analysis of Mouse brain lysate showing detection of ~67.5 kDa RYK protein using Rabbit Anti-RYK Polyclonal Antibody (SPC-740). Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse brain lysate. Load: 15 µg. Block: 5% Skim Milk in TBST. Primary Antibody: Rabbit Anti-RYK Polyclonal Antibody (SPC-740) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:4000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~67.5 kDa. Other Band(s): ~37 kDa degradation product.
    detail
  •  - RYK Antibody ASM10589
    Immunohistochemistry analysis using Rabbit Anti-RYK Polyclonal Antibody (SPC-740). Tissue: Breast Cancer. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-RYK Polyclonal Antibody (SPC-740) at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 10X. HRP-DAB Detection. | Western blot analysis of Mouse brain lysate showing detection of ~67.5 kDa RYK protein using Rabbit Anti-RYK Polyclonal Antibody (SPC-740). Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse brain lysate. Load: 15 µg. Block: 5% Skim Milk in TBST. Primary Antibody: Rabbit Anti-RYK Polyclonal Antibody (SPC-740) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:4000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~67.5 kDa. Other Band(s): ~37 kDa degradation product.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
IHC
Primary Accession P34925
Other Accession NP_001005861.1
Host Rabbit
Reactivity Rat
Clonality Polyclonal
Format RYK
Target/Specificity RYK
Other Names Tyrosine protein kinase RYK precursor Antibody, D3S3195 Antibody, Vik Antibody, Tyrosine protein kinase RYK Antibody, JTK5A protein tyrosine kinase Antibody, RYK1 Antibody, RYK 1 Antibody, receptor like tyrosine kinase precursor Antibody, Tyrosine-protein kinase RYK Antibody, receptor like tyrosine kinase Antibody, ERK 3 Antibody, RYK Antibody, JTK 5A Antibody, Hydroxyaryl protein kinase Antibody, JTK5 Antibody, JTK5A Antibody, RYK_HUMAN Antibody, Ryk Antibody, JTK 5 Antibody
Clone Names RYK
Immunogen Synthetic peptide of Human RYK (150-250 aa), conjugated to Keyhole Limpet Haemocyanin (KLH).
Purification Peptide Affinity Purified
Storage -20ºC
Storage Buffer PBS pH 7.4, 50% glycerol, 0.09% sodium azide *Storage buffer may change when conjugated
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis A 1:1000 dilution of SPC-740 was sufficient for detection of RYK in 15 µg of mouse brain cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Cellular Localization Membrane | Single-Pass Type I Membrane Protein | Nucleus | Cytoplasm
Research Areas
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$ 330.00
Cat# ASM10589
11 Formats Available
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