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SMAD4 Antibody
Rabbit Anti-Human SMAD4 Polyclonal
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| IHC |
|---|---|
| Primary Accession | Q13485 |
| Other Accession | NP_005350.1 |
| Host | Rabbit |
| Clonality | Polyclonal |
| Format | SMAD4 |
| Target/Specificity | SMAD4 |
| Other Names | SMAD 4 Antibody, MADH4 Antibody, SMAD4 Antibody, SMAD family member 4 Antibody, Smad4 Antibody, Mothers against decapentaplegic homolog 4 Antibody, DPC4 Antibody, hSMAD4 Antibody, MAD homolog 4 Antibody, Deletion target in pancreatic carcinoma 4 Antibody, Mothers against DPP homolog 4 Antibody |
| Clone Names | SMAD4 |
| Immunogen | Synthetic peptide of Human SMAD4 (200-300 aa), conjugated to Keyhole Limpet Haemocyanin (KLH). |
| Purification | Peptide Affinity Purified |
| Storage | -20ºC |
| Storage Buffer | PBS pH 7.4, 50% glycerol, 0.09% sodium azide *Storage buffer may change when conjugated |
| Shipping Temperature | Blue Ice or 4ºC |
| Certificate of Analysis | A 1:1000 dilution of SPC-730 was sufficient for detection of SMAD4 in 15 µg of human HeLa cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody. |
| Cellular Localization | Cytoplasm | Nucleus |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
Members of the Smad family of cell signaling molecules are components of a critical intracellular pathway that transmit TGF-β signals from the cell surface into the nucleus. There are three distinct classes of Smads: the receptor-regulated Smads (R-Smads), the common-mediator Smad (co-Smad), and the antagonistic or inhibitory Smads. Following stimulation by TGF-β, Smad2 and Smad3 become phosphorylated at their carboxyl termini (Ser465 and 467 on Smad2; Ser423 and 425 on Smad3) by TGF-β Receptor I. Phosphorylated Smad 2/3 can complex with Smad4, translocate to the nucleus and regulate gene expression.
References
1. Heldin C.H. et al. (1997) Nature. 390: 465-471.
2. Attisano L. and Wrana, J.L. (1998) Curr Opin Cell Biol. 10: 188-194.
3. Massagué J. (1998) Annu Rev Biochem. 67: 753-791.
4. Whitman M. (1998) Genes Dev. 12: 2445-2462.
5. Wu G. et al. (2000) Science. 287: 92-97.
6. Abdollah S., et al. (1997) J. Biol. Chem. 272: 27678-27685.
7. Souchelnytskyi S., et al. (1997) J. Biol. Chem. 272: 28107-28115.
8. Liu X., et al. (1997) Proc. Natl. Acad. Sci. USA. 94: 10669-10674.
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