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SMAD4 Antibody

Rabbit Anti-Human SMAD4 Polyclonal

     
  •  - SMAD4 Antibody ASM10579
    Immunohistochemistry analysis using Rabbit Anti-SMAD4 Polyclonal Antibody (SPC-730). Tissue: Breast Cancer. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-SMAD4 Polyclonal Antibody (SPC-730) at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 10X. HRP-DAB Detection. | Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~60.4 kDa SMAD4 protein using Rabbit Anti-SMAD4 Polyclonal Antibody (SPC-730). Lane 1: Molecular Weight Ladder (MW). Lane 2: Cervical Cancer cell line (HeLa) lysate. Load: 10 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-SMAD4 Polyclonal Antibody (SPC-730) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~60.4 kDa.
    detail
  •  - SMAD4 Antibody ASM10579
    Immunohistochemistry analysis using Rabbit Anti-SMAD4 Polyclonal Antibody (SPC-730). Tissue: Breast Cancer. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-SMAD4 Polyclonal Antibody (SPC-730) at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 10X. HRP-DAB Detection. | Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~60.4 kDa SMAD4 protein using Rabbit Anti-SMAD4 Polyclonal Antibody (SPC-730). Lane 1: Molecular Weight Ladder (MW). Lane 2: Cervical Cancer cell line (HeLa) lysate. Load: 10 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-SMAD4 Polyclonal Antibody (SPC-730) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~60.4 kDa.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
IHC
Primary Accession Q13485
Other Accession NP_005350.1
Host Rabbit
Clonality Polyclonal
Format SMAD4
Target/Specificity SMAD4
Other Names SMAD 4 Antibody, MADH4 Antibody, SMAD4 Antibody, SMAD family member 4 Antibody, Smad4 Antibody, Mothers against decapentaplegic homolog 4 Antibody, DPC4 Antibody, hSMAD4 Antibody, MAD homolog 4 Antibody, Deletion target in pancreatic carcinoma 4 Antibody, Mothers against DPP homolog 4 Antibody
Clone Names SMAD4
Immunogen Synthetic peptide of Human SMAD4 (200-300 aa), conjugated to Keyhole Limpet Haemocyanin (KLH).
Purification Peptide Affinity Purified
Storage -20ºC
Storage Buffer PBS pH 7.4, 50% glycerol, 0.09% sodium azide *Storage buffer may change when conjugated
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis A 1:1000 dilution of SPC-730 was sufficient for detection of SMAD4 in 15 µg of human HeLa cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Cellular Localization Cytoplasm | Nucleus
Research Areas
Citations (0)
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Background

Members of the Smad family of cell signaling molecules are components of a critical intracellular pathway that transmit TGF-β signals from the cell surface into the nucleus. There are three distinct classes of Smads: the receptor-regulated Smads (R-Smads), the common-mediator Smad (co-Smad), and the antagonistic or inhibitory Smads. Following stimulation by TGF-β, Smad2 and Smad3 become phosphorylated at their carboxyl termini (Ser465 and 467 on Smad2; Ser423 and 425 on Smad3) by TGF-β Receptor I. Phosphorylated Smad 2/3 can complex with Smad4, translocate to the nucleus and regulate gene expression.

References

1. Heldin C.H. et al. (1997) Nature. 390: 465-471.
2. Attisano L. and Wrana, J.L. (1998) Curr Opin Cell Biol. 10: 188-194.
3. Massagué J. (1998) Annu Rev Biochem. 67: 753-791.
4. Whitman M. (1998) Genes Dev. 12: 2445-2462.
5. Wu G. et al. (2000) Science. 287: 92-97.
6. Abdollah S., et al. (1997) J. Biol. Chem. 272: 27678-27685.
7. Souchelnytskyi S., et al. (1997) J. Biol. Chem. 272: 28107-28115.
8. Liu X., et al. (1997) Proc. Natl. Acad. Sci. USA. 94: 10669-10674.

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$ 330.00
Cat# ASM10579
11 Formats Available
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