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COVID19
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c-Jun Antibody

Rabbit Anti-Human c-Jun Polyclonal

     
  •  - c-Jun Antibody ASM10577
    Immunohistochemistry analysis using Rabbit Anti-c-Jun Polyclonal Antibody (SPC-728). Tissue: Breast Cancer. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-c-Jun Polyclonal Antibody (SPC-728) at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Localization: Nucleus. Magnification: 10X. HRP-DAB Detection. | Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~35.7 kDa c-Jun protein using Rabbit Anti-c-Jun Polyclonal Antibody (SPC-728). Lane 1: Molecular Weight Ladder (MW). Lane 2: Cervical Cancer cell line (HeLa) lysate. Load: 10 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-c-Jun Polyclonal Antibody (SPC-728) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~35.7 kDa. Other Band(s): ~25 kDa potential degradation product.
    detail
  •  - c-Jun Antibody ASM10577
    Immunohistochemistry analysis using Rabbit Anti-c-Jun Polyclonal Antibody (SPC-728). Tissue: Breast Cancer. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-c-Jun Polyclonal Antibody (SPC-728) at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Localization: Nucleus. Magnification: 10X. HRP-DAB Detection. | Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~35.7 kDa c-Jun protein using Rabbit Anti-c-Jun Polyclonal Antibody (SPC-728). Lane 1: Molecular Weight Ladder (MW). Lane 2: Cervical Cancer cell line (HeLa) lysate. Load: 10 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-c-Jun Polyclonal Antibody (SPC-728) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~35.7 kDa. Other Band(s): ~25 kDa potential degradation product.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
IHC
Primary Accession P05412
Other Accession NP_002219.1
Host Rabbit
Clonality Polyclonal
Format c-Jun
Target/Specificity c-Jun
Other Names AP 1 Antibody, Proto-oncogene c-Jun Antibody, cJun Antibody, Enhancer Binding Protein AP1 Antibody, p39 Antibody, JUN Antibody, JUN_HUMAN Antibody, V jun avian sarcoma virus 17 oncogene homolog Antibody, Jun Activation Domain Binding Protein Antibody, JUNC Antibody, Transcription Factor AP 1 Antibody, Oncogene JUN Antibody, V-jun avian sarcoma virus 17 oncogene homolog Antibody, Jun proto oncogene Antibody, Proto oncogene c jun Antibody, Transcription Factor AP1 Antibody, V jun sarcoma virus 17 oncogene homolog Antibody, Proto-oncogene c-jun Antibody, Proto oncogene cJun Antibody, V jun sarcoma virus 17 oncogene homolog (avian) Antibody, AP1 Antibody, Activator protein 1 Antibody, JUN protein Antibody, Jun oncogene Antibody, Transcription factor AP-1 Antibody, vJun Avian Sarcoma Virus 17 Oncogene Homolog Antibody
Clone Names c-Jun
Immunogen Synthetic peptide of Human c-Jun (200-300 aa), conjugated to Keyhole Limpet Haemocyanin (KLH).
Purification Peptide Affinity Purified
Storage -20ºC
Storage Buffer PBS pH 7.4, 50% glycerol, 0.09% sodium azide *Storage buffer may change when conjugated
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis A 1:1000 dilution of SPC-728 was sufficient for detection of c-Jun in 15 µg of human HeLa cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Cellular Localization Nucleus
Research Areas
Citations (0)
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$ 330.00
Cat# ASM10577
11 Formats Available
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