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NFATC1 antibody

Rabbit Anti-Human NFATC1 Polyclonal

     
  •  - NFATC1 antibody ASM10576
    Immunohistochemistry analysis using Rabbit Anti-NFATC1 Polyclonal Antibody (SPC-727). Tissue: Spleen. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-NFATC1 Polyclonal Antibody (SPC-727) at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 10X. | Western blot analysis of Rat brain lysate showing detection of ~101.2 kDa NFATC1 protein using Rabbit Anti-NFATC1 Polyclonal Antibody (SPC-727). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat brain lysate. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-NFATC1 Polyclonal Antibody (SPC-727) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~101.2 kDa. Other Band(s): ~60 kDa isoform.
    detail
  •  - NFATC1 antibody ASM10576
    Immunohistochemistry analysis using Rabbit Anti-NFATC1 Polyclonal Antibody (SPC-727). Tissue: Spleen. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-NFATC1 Polyclonal Antibody (SPC-727) at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 10X. | Western blot analysis of Rat brain lysate showing detection of ~101.2 kDa NFATC1 protein using Rabbit Anti-NFATC1 Polyclonal Antibody (SPC-727). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat brain lysate. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-NFATC1 Polyclonal Antibody (SPC-727) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~101.2 kDa. Other Band(s): ~60 kDa isoform.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
IHC
Primary Accession O95644
Other Accession NP_001265598.1
Host Rabbit
Reactivity Rat
Clonality Polyclonal
Format NFATC1
Target/Specificity NFATC1
Other Names NFAT2 Antibody, NFATc Antibody, OR56B4 Antibody, Olfactory receptor OR11-67 Antibody, NF-ATc1 Antibody, OR11-67 Antibody, NFAT transcription complex cytosolic component Antibody, NFATc1 Antibody, NF-ATc Antibody, Nuclear factor of activated T-cells cytoplasmic 1 Antibody, NFATC Antibody, NFATC1 Antibody
Clone Names NFATC1
Immunogen Synthetic peptide of Human NFATC1 (110-210 aa), conjugated to Keyhole Limpet Haemocyanin (KLH).
Purification Peptide Affinity Purified
Storage -20ºC
Storage Buffer PBS pH 7.4, 50% glycerol, 0.09% sodium azide *Storage buffer may change when conjugated
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis A 1:1000 dilution of SPC-727 was sufficient for detection of NFATC1 in 15 µg of rat brain cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Cellular Localization Cytoplasm | Nucleus
Research Areas
Citations (0)
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$ 330.00
Cat# ASM10576
11 Formats Available
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