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Axin 1 Antibody

Rabbit Anti-Human Axin 1 Polyclonal

     
  •  - Axin 1 Antibody ASM10570
    Western blot analysis of Mouse kidney lysate showing detection of ~95.6 kDa Axin 1 protein using Rabbit Anti-Axin 1 Polyclonal Antibody (SPC-721). Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse kidney lysate. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Axin 1 Polyclonal Antibody (SPC-721) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~95.6 kDa.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession O15169
Other Accession NP_003493.1
Host Rabbit
Clonality Polyclonal
Format Axin 1
Target/Specificity Axin 1
Other Names AXIN1 Antibody, hAxin Antibody, Axin-1 Antibody, Axis inhibition protein 1 Antibody, AXIN Antibody, Escherichia coli O119 Antibody
Clone Names Axin 1
Immunogen Synthetic peptide of Human Axin 1 (200-300 aa), conjugated to Keyhole Limpet Haemocyanin (KLH).
Purification Peptide Affinity Purified
Storage -20ºC
Storage Buffer PBS pH 7.4, 50% glycerol, 0.09% sodium azide *Storage buffer may change when conjugated
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis A 1:1000 dilution of SPC-721 was sufficient for detection of Axin 1 in 15 µg of mouse kidney cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Cellular Localization Cytoplasm | Nucleus | Membrane | Cell Membrane
Research Areas
Citations (0)
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$ 330.00
Cat# ASM10570
11 Formats Available
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