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HSF2 Antibody

     
  •  - HSF2 Antibody ASM10565
    Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-HSF2 Polyclonal Antibody (ASM10565). Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-HSF2 Polyclonal Antibody (ASM10565) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasmic. Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) HSF2 Antibody. (D) Composite.
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  • WB - HSF2 Antibody ASM10565
    Western blot analysis of Rat Liver cell lysates showing detection of ~60.3 kDa HSF2 protein using Rabbit Anti-HSF2 Polyclonal Antibody (ASM10565). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Liver cell lysates. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-HSF2 Polyclonal Antibody (ASM10565) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~60.3 kDa.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, ICC
Primary Accession Q03933
Other Accession NP_001129036.1
Host Rabbit
Reactivity Rat
Clonality Polyclonal
Description Rabbit Anti-Human HSF2 Polyclonal
Target/Specificity Detects ~90 kDa.
Other Names Heat shock factor protein 2 Antibody, Heat shock transcription factor 2 Antibody, HSTF 2 Antibody, HSTF2 Antibody, HSF 2 Antibody, HSF2 Antibody, HSF2_HUMAN Antibody
Immunogen Synthetic peptide from the mid-protein of Human HSF2
Purification Peptide Affinity Purified
Storage -20ºC
Storage Buffer PBS, 50% glycerol, 0.09% sodium azide
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis A 1:1000 dilution of SPC-712 was sufficient for detection of HSF2 in 15 µg of rat liver cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Cellular Localization Cytoplasm | Nucleus
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Cat# ASM10565
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