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RIP3 Antibody

     
  •  - RIP3 Antibody ASM10538
    Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-RIP3 Polyclonal Antibody (ASM10538). Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-RIP3 Polyclonal Antibody (ASM10538) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) RIP3 Antibody. (D) Composite.
    detail
  • WB - RIP3 Antibody ASM10538
    Western blot analysis of Mouse Brain cell lysates showing detection of ~56.9 kDa RIP3 protein using Rabbit Anti-RIP3 Polyclonal Antibody (ASM10538). Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Brain cell lysates. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-RIP3 Polyclonal Antibody (ASM10538) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:1000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~56.9 kDa.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, ICC
Primary Accession Q9Y572
Other Accession NP_006862.2
Host Rabbit
Reactivity Human, Mouse
Clonality Polyclonal
Description Rabbit Anti-Human RIP3 Polyclonal
Target/Specificity Detects ~57 kDa.
Other Names RIPK3 Antibody, Receptor-interacting protein 3 Antibody, Receptor interacting protein 3 Antibody, Receptor interacting serine/threonine protein kinase 3 Antibody, RIP-3 Antibody, RIPK3_HUMAN Antibody, Receptor-interacting serine/threonine-protein kinase 3 Antibody, RIPK 3 Antibody, RIP 3 Antibody, Receptor interacting serine threonine kinase 3 Antibody, RIP3 Antibody, RIP like protein kinase 3 Antibody, RIP-like protein kinase 3 Antibody
Immunogen Synthetic peptide from the N-terminal to middle of Human RIP3
Purification Peptide Affinity Purified
Storage -20ºC
Storage Buffer PBS, 50% glycerol, 0.09% sodium azide
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis A 1:1000 dilution of SPC-672 was sufficient for detection of RIP3 in 15 µg of mouse brain cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Cellular Localization Cytoplasm | Cytosol | Cell Membrane | Mitochondrion
Research Areas
Citations (0)
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Cat# ASM10538
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