ATG7 Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, ICC |
---|---|
Primary Accession | O95352 |
Other Accession | NP_001129503.2 |
Host | Rabbit |
Reactivity | Human, Mouse, Rat |
Clonality | Polyclonal |
Description | Rabbit Anti-Human ATG7 Polyclonal |
Target/Specificity | Detects 77.9 kDa. |
Other Names | Autophagy 7S. cerevisiae, homolog of Antibody, APG7 like Antibody, ATG7_HUMAN Antibody, hAGP7 Antibody, Ubiquitin-like modifier-activating enzyme ATG7 Antibody, Autophagy related protein 7 Antibody, Autophagy-related protein 7 Antibody, GSA 7 Antibody, APG7, S. cerevisiae, homolog of Antibody, Ubiquitin-activating enzyme E1-like protein Antibody, Autophagy-related 7 (yeast) Antibody, APG7 autophagy 7 like Antibody, 1810013K23Rik Antibody, APG7 autophagy 7-like (S. cerevisiae) Antibody, ATG12-activating enzyme E1 ATG7 Antibody, ATG7 autophagy related 7 homolog Antibody, Atg7l Antibody, Ubiquitin activating enzyme E1 like protein Antibody, Apg 7 Antibody, APG7L Antibody, DKFZp434N0735 Antibody, ATG 7 Antibody, APG7-like Antibody, GSA7 Antibody, ATG7 autophagy related 7 homolog (S. cerevisiae) Antibody, ATG7 Antibody |
Immunogen | Synthetic peptide from the N-terminal of Human ATG7 |
Purification | Peptide Affinity Purified |
Storage | -20ºC |
Storage Buffer | PBS, 50% glycerol, 0.09% sodium azide |
Shipping Temperature | Blue Ice or 4ºC |
Certificate of Analysis | A 1:1000 dilution of SPC-610 was sufficient for detection of ATG7 in 15 µg of human HeLa cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody. |
Cellular Localization | Cytoplasm | Preautophagosomal Structure |
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Provided below are standard protocols that you may find useful for product applications.
Background
ATG7 in conjuction with ATG10, mediates the formation of the autophagosome when ATG12 is covalently bound to ATG5 and targets to autophagosome vesicles. It also activates ATG8, and is crucial for amino acid supply in neonates.
References
1. Mizushima N., et al. (1998) J Biol Chem. 273: 33889-92.
2. Mizushima N., et al. (1998) Nature. 395: 395-8.
3. Suzuki K., et al. (2001) EMBO J. 20: 5971-81.
4. Tanida I., et al. (1999) Mol Biol Cell. 10: 1367-79.
5. Shintani T., et al. (1999) EMBO J. 18: 5234-41.
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